Human Anti-Mouse IgG Abs




Test Mnemonic

MOUABS

CPT Codes

  • 83520 - QTY (1)

LOINC ®

14975-7

Aliases

  • HAMA

Includes

  • Human Anti-Mouse Ab (HAMA), by ELISA

Performing Laboratory

ARUP


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mLSerumNo additive (Red) FrozenDo not use gel separator tubes. Separate specimens must be submitted when multiple tests are ordered.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
0.5 mL     

Stability

Environmental Condition Description
AmbientUnacceptable
Refrigerated24 hours
Frozen21 days

Days Performed

Varies

Turnaround Time

13 - 19 days

Methodology

Name Description
Enzyme-Linked Immunosorbent Assay (ELISA) 

Reference Range

Special Info

Do not use gel separator tubes. Separate specimens must be submitted when multiple tests are ordered. This test is New York state approved.

Clinical Info

The human anti-mouse antibodies (HAMA) direct ELISA test is intended for the determination of IgG subclass of isotypic HAMA in human serum. HAMA have been reported to give false positive results in two-site immunometric assays that utilize murine (mouse) monoclonal IgG. HAMA reactivity has been detected in approximately 9% of a normal population without known exposure to murine IgG. Such responses may be due to polyclonal rheumatoid factors, heterophilic antibodies, dietary, or other exposure. Therapeutic use of murine monoclonal antibodies (IgG) or their fragments either unmodified or conjugated to drugs, toxins or radionucleotides may induce immune response directed against the same IgG and produce a significant level of HAMA in serum. Circulating levels of HAMA can bind to the injected IgG and reduce the efficacy of the antibody therapy. HAMA also can cause anaphylactic complications to subsequent administration of murine monoclonal IgG.