Immunodeficiency (CDC) Panel




Test Mnemonic

IMMDEF

CPT Codes

  • 86357 - QTY (1)
  • 86359 - QTY (1)
  • 86360 - QTY (1)
  • 86355 - QTY (1)

Aliases

  • Lymphocyte Subsets

Includes

  • CD3+ T Cells
  • CD3+CD4+ T Cells
  • CD3+CD8+ T Cells
  • CD19+ B Cells
  • CD3-CD16/56+ NK Cells
  • CD4/CD8 Ratio

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
4 mLWhole bloodEDTA (Lavender)AmbientAmbientDeliver specimens to testing laboratory within 24 hours of collection.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mL     

Stability

Environmental Condition Description
AmbientDeliver specimens to testing laboratory within 24 hours of collection.
RefrigeratedUnacceptable
FrozenUnacceptable

Days Performed

Mon - Sat

Turnaround Time

1 - 2 days

Reference Range

CD19+ B Cell %
Sex Age From Age To Type Range Range Unit
 5 Years99 YearsNormal5 - 22%
CD19+ B Cell No.
Sex Age From Age To Type Range Range Unit
 5 Years99 YearsNormal75 - 660cells/uL
CD3+ T Cell No.
Sex Age From Age To Type Range Range Unit
   Months3 MonthsNormal2070 - 6540cells/uL
 4 Months11 MonthsNormal2280 - 6450cells/uL
 12 Months23 MonthsNormal1460 - 5440cells/uL
 24 Months59 MonthsNormal1610 - 4230cells/uL
 5 Years99 YearsNormal958 - 2388cells/uL
CD3+CD8+ T Cell No.
Sex Age From Age To Type Range Range Unit
   Months3 MonthsNormal650 - 2450cells/uL
 4 Months11 MonthsNormal720 - 2490cells/uL
 12 Months23 MonthsNormal570 - 2230cells/uL
 24 Months59 MonthsNormal630 - 1910cells/uL
 5 Years99 YearsNormal175 - 958cells/uL
CD4/CD8 Ratio
Sex Age From Age To Type Range Range Unit
   Months3 MonthsNormal1.32 - 3.47.
 4 Months11 MonthsNormal1.20 - 3.48.
 12 Months23 MonthsNormal0.95 - 2.95.
 24 Months59 MonthsNormal1.05 - 2.07.
 5 Years99 YearsNormal1.10 - 3.25.
CD4+CD3+ T Cell %
Sex Age From Age To Type Range Range Unit
   Months3 MonthsNormal41 - 64%
 4 Months11 MonthsNormal36 - 61%
 12 Months23 MonthsNormal31 - 54%
 24 Months59 MonthsNormal25 - 51%
 5 Years99 YearsNormal34 - 61%
CD4+CD3+ T Cell No.
Sex Age From Age To Type Range Range Unit
   Months3 MonthsNormal1460 - 5110cells/uL
 4 Months11 MonthsNormal1690 - 4600cells/uL
 12 Months23 MonthsNormal1020 - 3600cells/uL
 24 Months59 MonthsNormal900 - 2860cells/uL
 5 Years99 YearsNormal533 - 1674cells/uL
NK Cell %
Sex Age From Age To Type Range Range Unit
 5 Years99 YearsNormal102 - 565cells/uL
 5 Years99 YearsNormal5 - 25%

Special Info

Samples received between 24 and 48 hours of draw time will be performed and reported along with a lymphocyte viability. Samples received between 48 and 72 hours old will performed and reported along with a lymphocyte viability; however, NK results may not be accurate. Recommend obtaining a fresh sample if clinically indicated. Samples that are less than 75% viable will be rejected and a redraw will be requested. Samples received in testing lab beyond 72 hours cannot be performed. Refrigerated samples may give abberant results and will be rejected.

Clinical Info

This is a single platform, 6-color Flow Cytometry assay used to enumerate CD3+ T cells; CD3+CD4+ T cells; CD3+CD8+ T cells; CD19+ B cells; CD3-CD16/56+ NK cells; and CD4/CD8 Ratio in peripheral blood. These lymphocyte subset percentages and counts are used in the monitoring of Immunodeficiency, HIV infection, and other immunologic disorders.