Insulin, Free and Total, Serum
Test Mnemonic
INSFT
CPT Codes
- 83527 - QTY (1)
- 83525 - QTY (1)
Includes
- Free Insulin
- Total Insulin
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Serum | SST (Gold) | Refrigerated |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.5 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 24 hours |
| Refrigerated | 7 days |
| Frozen | 30 days |
Days Performed
2 days per week
Turnaround Time
1 - 4 days
Methodology
| Name | Description |
|---|---|
| Electro Chemiluminescence Immunoassay (ECLIA) |
Reference Range
| Free Insulin | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 2.6 - 24.9 | uU/mL | Reference intervals established for fasting specimens | ||
| Total Insulin | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 2.6 - 24.9 | uU/mL | Reference intervals established for fasting specimens |
Special Info
Specimen collection should occur preferably from patients fasting for at least 8 hours and at minimum 8 hours after administration of multivitamins or dietary supplements, especially containing biotin (Vitamin B7).
Clinical Info
Intended for the in vitro quantitative determination of human insulin in human serum and plasma. The determination of insulin is utilized in the diagnosis and therapy of various disorders of carbohydrate metabolism, including diabetes mellitus and hypoglycemia.
Clinical Limitation
For diagnostic purposes, the results should always be assessed in conjunction with the patient’s medical history, clinical examination and other findings.