JC Polyoma Virus Quantitative PCR
Test Mnemonic
JCQNT
CPT Codes
- 87799 - QTY (1)
Aliases
- JC Virus Quant PCR
Performing Laboratory
Focus Laboratories MRL
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.7 mL | Plasma | EDTA (Lavender) | Frozen | Separate plasma from cells within 2 hours of collection, and transfer into a sterile aliquot tube. Do not clarify plasma by filtration or further centrifugation. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.7 mL | Plasma | EDTA PPT (White) | Frozen | Centrifuge within 2 hours of collection. The plasma is not required to be transferred to an aliquot tube. | |
| 0.7 mL | Plasma | ACD A (Yellow) | Frozen | Separate plasma from cells within 2 hours of collection, and transfer into a sterile aliquot tube. Do not clarify plasma by filtration or further centrifugation. | |
| 0.7 mL | Serum | SST (Gold) | Frozen | Allow blood to clot at room temperature. Separate serum from cells within 1 hour of collection, and transfer into a sterile aliquot tube. | |
| 0.7 mL | Serum | No additive (Red) | Frozen | Allow blood to clot at room temperature. Separate serum from cells within 1 hour of collection, and transfer into a sterile aliquot tube. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.3 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Frozen | 30 days (Avoid repeated freeze/thaw cycles) |
| Refrigerated | 7 days |
| Ambient | 48 hours |
Days Performed
Mon - Sat
Turnaround Time
2 - 4 days
Methodology
| Name | Description |
|---|---|
| Real-Time Polymerase Chain Reaction (RT-PCR) |
Reference Range
| JC Virus DNA, Quant PCR | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Years | 99 Years | Normal | < 500 | copies/mL |
Special Info
Avoid repeated freezing and thawing of specimens. The published serum or plasma specimen types are the only acceptable specimen types; all other specimens will be rejected.
Clinical Info
JC virus is the cause of progressive multifocal leukoencephalopathy (PML), a severe demyelinating disease of the central nervous system. PML is a particular concern to individuals infected with the human immunodeficiency virus. Quantification of JC virus DNA is based upon real-time PCR amplification and detection of JCV genomic DNA. The quantitative range of the assay is 500 - 35,000,000 JCV DNA copies/mL.