LH, Pediatric
Test Mnemonic
LHPED
CPT Codes
- 83002 - QTY (1)
LOINC ®
10501-5
Aliases
- Luteinizing Hormone, Pediatric
Includes
- LH, Pediatrics, S
Performing Laboratory
Mayo Clinic Dpt of Lab Med & Pathology
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.25 mL | Serum | No additive (Red) | Refrigerated | Separate serum from cells ASAP or within 2 hours of collection. Transfer serum to standard aliquot tube. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.25 mL | Serum | SST (Gold) | Refrigerated | Separate serum from cells ASAP or within 2 hours of collection. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.15 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | After separation from cells: Unacceptable |
| Refrigerated | After separation from cells: 14 days |
| Frozen | After separation from cells: 90 days |
Days Performed
Tue, Thu
Turnaround Time
3 - 7 days
Methodology
| Name | Description |
|---|---|
| Chemiluminescence Immunoassay (CLIA) |
Reference Range
| LH, Pediatric | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Female | Days | 364 Days | Normal | <0.02-18.3 | IU/L | Female | 1 Years | 8 Years | Normal | <0.02-0.3 | IU/L | Female | 9 Years | 10 Years | Normal | <0.02-4.8 | IU/L | Female | 11 Years | 13 Years | Normal | <0.02-11.7 | IU/L | Female | 14 Years | 17 Years | Normal | <0.02-16.7 | IU/L | Female | Normal | Tanner Stage I (1-8 years): <0.02-0.3 | IU/L | Female | Normal | Tanner Stage II: <0.02-4.1 | IU/L | Female | Normal | Tanner Stage IIl: 0.6-7.2 | IU/L | Female | Normal | Tanner Stage IV-V: 0.9-13.3 | IU/L | Male | Days | 364 Days | Normal | <0.02-5.0 | IU/L | Male | 1 Years | 8 Years | Normal | <0.02-0.5 | IU/L | Male | 9 Years | 10 Years | Normal | <0.02-3.6 | IU/L | Male | 11 Years | 13 Years | Normal | 0.1-5.7 | IU/L | Male | 14 Years | 17 Years | Normal | 0.8-8.7 | IU/L | Male | Normal | Tanner Stage I (1-8 years): <0.02-0.5 | IU/L | Male | Normal | Tanner Stage II: 0.03-3.7 | IU/L | Male | Normal | Tanner Stage IIl: 0.09-4.2 | IU/L | Male | Normal | Tanner Stage IV-V: 1.3-9.8 | IU/L |
Special Info
Grossly hemolyzed, lipemic or icteric specimens will be rejected. This test is New York DOH approved.
Clinical Info
This test is useful for diagnosis of precocious puberty and delayed puberty in children. Luteinizing hormone (LH) is a glycoprotein hormone consisting of 2 noncovalently bound subunits (alpha and beta). LH is produced by the anterior pituitary gland under regulation of the hypothalamic gonadotropin releasing hormone (GnRH) and feedback from gonadal steroid hormones. In children, LH, along with follicle-stimulating hormone (FSH), is used to diagnose precocious (early) and delayed puberty. Precocious puberty refers to the appearance of physical and hormonal signs of pubertal development at an earlier age than is considered normal (before 8 years of age in girls and 9 years of age in boys). Evaluation of precocious puberty includes measurement of LH and FSH to determine whether gonadotropins are increased in relation to chronologic age (gonadotropin-dependent) or whether sex steroid secretion is occurring independent of LH and FSH (gonadotropin-independent). In gonadotropin-dependent precocious puberty, basal LH levels are often elevated into the pubertal range and show a pubertal (heightened) response to GnRH stimulation. In gonadotropin-independent precocious puberty, the LH level is low at baseline and fails to respond to GnRH stimulation. Delayed puberty is defined clinically by the absence or incomplete development of secondary sexual characteristics by age 14 years in boys and by age 12 years in girls. Delayed puberty usually results from inadequate gonadal steroid secretion that, in turn, is most often caused by a defective gonadotropin secretion from the anterior pituitary, due to defective production of GnRH from the hypothalamus. Random measurements of LH and FSH, together with estradiol (females) or testosterone (males), are useful to distinguish between primary and secondary causes of delayed puberty. This assay is sensitive to 0.02 IU/L and is more appropriate for use in children.
Clinical Limitation
Cross-reactivity with thyroid-stimulating hormone (TSH) (<5%) might be observed at TSH concentrations of 500 mIU/L. Some patients who have been exposed to animal antigens, either in the environment or as part of treatment or imaging procedures, may have circulating anti-animal antibodies present. These antibodies may interfere with the assay reagents to produce unreliable results.