Lupus Anticoagulant Diagnostic Interpretive Panel




Test Mnemonic

LUPUSP

CPT Codes

  • 85670 - QTY (1)
  • 86147 - QTY (3)
  • 85610 - QTY (1)
  • 85390 - QTY (1)
  • 86146 - QTY (2)
  • 85520 - QTY (1)
  • 85730 - QTY (1)
  • 85730 - QTY (1)
  • 85598-variable - QTY (1)

Aliases

  • Lupus Anticoag Panel

Includes

  • APTT
  • Anticardiolipin Ab IgG, IgM and IgA
  • Beta 2 Glycoproteins IgG and IgM
  • Prothrombin Time (PT)
  • APTT Screen
  • Thrombin Time
  • Anti Xa Inhibitor Assay
  • Dilute Russell Viper Venom Time (DRVVT)
  • Hexagonal Phase Phospholipid Neutralization (Staclot)
  • Platelet Neutralization (PNP)

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mLSerumSST (Gold) Frozen 
5 mLPlasmaSodium citrate (Lt. Blue) Centrifuge, aliquot and freeze ASAP.Collection tubes must be filled to total fill volume. Inadequately filled tubes will be rejected. Non-testing sites: Centrifuge samples; Aliquot plasma into a separate tube and label with Epic Beaker labels. Specimens should be frozen (-20C or colder). Indicate on each tube as plasma.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
0.2 mL    Serum
2.5 mL    Plasma

Stability

Environmental Condition Description
Ambient4 hours - Main campus: ACCEPTABLE for Whole Blood. (Must be delivered ambient to testing lab less than 4 hours post collection.) Non-Testing Sites: UNACCEPTABLE.
RefrigeratedUnacceptable
FrozenFor Non-Testing sites, Frozen Plasma is ACCEPTABLE. (Centrifuge samples, then aliquot plasma into a separate tube and label with Epic Beaker labels. Specimens should be frozen at -20° C and they are stable for 2 weeks. Specimens frozen at -70° C are stable for up to 6 months.

Days Performed

Mon - Fri

Turnaround Time

3 - 5 days

Methodology

Name Description
Refer to individual components 

Reference Range

Special Info

3.2% sodium citrate is the preferred anticoagulant recommended by NCCLS. Patient preparation: Discontinue heparin therapy for 2 days prior to collection. If tests are abnormal, the following tests may be ordered and billed: PTT Mixing Study (85730), Factor II (85210), Factor V (85220), Factor VII (85230), Factor X (85260), Factor VIII (85247), Von Willebrand Factor Antigen (85246), Ristocetin Co-factor (85245), Factor IX Assay (85250), Factor XI Assay (85270), Factor XII Assay (85280), Reptilase Time (85635), D-Dimer (85379), Fibrinogen Ag (85385), Fibrinogen (85384), Bethesda Assay (85335) and Factor VIII Chromogenic (85240). Sample must be accompanied by the completed Clinical History Form for Hemostasis and Thrombosis Evaluation.

Clinical Info

Evaluation of acquired hypercoagulable states and detection of Lupus anticoagulant and Antiphospholipid antibodies