MSI by PCR Analysis
Test Mnemonic
MSIID
CPT Codes
- 81301 - QTY (1)
Aliases
- Microsatellite Instability
- MSICCT
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 10 mm square | Block, Formalin fixed paraffin | Formalin-fixed, paraffin-embedded block | Ambient | Ambient | Formalin-fixed paraffin-embedded tissue slides. Transport and store slides at ambient temperature. Three unstained sections FFPE tissue on charged, unbaked slides or scrolls, plus one H&E stained section with best tumor area circled by a pathologist. Provide the percentage of tumor cells present, minimum 10%. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | FFPET slides and scrolls are stable at ambient temperature indefinitely |
| Refrigerated | FFPET slides and scrolls are stable refrigerated indefinitely |
| Frozen | Unacceptable |
Days Performed
Mon - Fri
Turnaround Time
3 days
Methodology
| Name | Description |
|---|---|
| Real-Time Polymerase Chain Reaction (RT-PCR) |
Special Info
Seven monomorphic biomarkers (ACVR2A, BTBD7, DIDO1, MRE11, RYR3, SEC31A AND SULF2) are analyzed to determine microsatellite status.
Clinical Info
The MSI PCR Analysis assay qualitatively identifies microsatellite instability (MSI-H), which is indicative of mismatch repair deficiency.
Clinical Limitation
Seven monomorphic biomarkers (ACVR2A, BTBD7, DIDO1, MRE11, RYR3, SEC31A AND SULF2) are analyzed to determine microsatellite status.