Neutrophil Oxidative Burst, Blood
Test Mnemonic
OXBRST
CPT Codes
- 86352 - QTY (1)
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3 mL | Whole blood | Sodium or Lithium heparin (Green) | See note | CRITICAL AMBIENT. THIS TEST REQUIRES MULTIPLE SPECIMENS. Send both specimens to Cleveland Clinic Laboratories on the day of collection by 3 p.m. EST. | |
| 3 mL | Whole blood | Sodium or Lithium heparin (Green) | See note | CRITICAL AMBIENT. THIS TEST REQUIRES MULTIPLE SPECIMENS. Collect this specimen from an unrelated healthy person; label the tube as "Control." Both tubes must be received in Cleveland Clinic Laboratories on the day of collection by 3 p.m. EST. Patient and control specimens must be collected within 48 hours of test performance. Do NOT refrigerate or freeze as live neutrophils are required. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| Other | Note: Minimum volume is 1 mL per tube (patient and control). Prefer 3 mL per tube. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 48 hours |
| Frozen | Unacceptable |
| Refrigerated | Unacceptable |
Days Performed
Sun - Sat
Turnaround Time
3 - 4 days
Methodology
| Name | Description |
|---|---|
| Semi-Quantiative Flow Cytometry |
Reference Range
Special Info
CRITICAL AMBIENT. Patient prep: Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient. Patient and control specimens MUST be collected within 48 hours of test performance. Do NOT refrigerate or freeze as live neutrophils are required. Ambient stability is 24 hours for New York clients. This test is New York DOH approved.
Clinical Info
Aids in screening for chronic granulomatous disease. White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by flow cytometry. Results are reported as the ratio of the mean channel fluorescence of stimulated cells versus unstimulated cells, which yields a stimulation index (SI). If sample shows abnormal results when stimulated, and no control was sent, test should be resubmitted with control sample to validate the conditions of collection, processing and transport. Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.