PML/RARA RTPCR
Test Mnemonic
APLPCR
CPT Codes
- 81315 - QTY (1)
Aliases
- PML-RARA Detection by RT-PCR, Quantitative
Includes
- PML-RARA Translocation Source
- PML-RARA Translocation
- PML-RARA Translocation Quant
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 5 mL | Whole blood | EDTA (Lavender) | Refrigerated | Critical refrigerated. Specimen must be delivered to Cleveland Clinic Laboratories by 3 p.m. EST on the day of collection. DO NOT collect the day before or the day of a major holiday. Separate specimens must be submitted when multiple tests are ordered. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3 mL | Bone marrow | EDTA (Lavender) | Refrigerated | Critical refrigerated. Specimen must be delivered to Cleveland Clinic Laboratories by 3 p.m. EST on the day of collection. DO NOT collect the day before or the day of a major holiday. Separate specimens must be submitted when multiple tests are ordered. | |
| Other | See note | See note | Frozen, Critical | Critical frozen. Transport 40 uL extracted RNA at a concentration of at least 40 ng/uL (minimum 40 uL) using a tissue transport kit (ARUP supply #47808). RNA must be extracted by a CLIA certified lab. Separate specimens must be submitted when multiple tests are ordered. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Bone marrow | ||||
| 3 mL | Whole blood |
Stability
| Environmental Condition | Description |
|---|---|
| Refrigerated | Blood, bone marrow: 48 hours; Extracted RNA: Unacceptable |
| Frozen | Blood, bone marrow: Unacceptable; Extracted RNA: Indefinitely |
| Ambient | Unacceptable |
Days Performed
Varies
Turnaround Time
3 - 10 days
Methodology
| Name | Description |
|---|---|
| Reverse Transcription/Polymerase Chain Reaction (RT/PCR) |
Reference Range
Special Info
Blood, bone marrow; CRITICAL REFRIGERATED. Extracted RNA: CRITICAL FROZEN. RNA isolation performed Sun - Sat. The following specimens are unacceptable: Severely hemolyzed or clotted specimens, serum, plasma, CSF, ambient bone marrow or whole blood, frozen bone marrow or whole blood, extracted DNA, RNA extracted by a non-CLIA lab, bone core, FFPE tissue, specimens collected in anticoagulants other than EDTA. This test is New York DOH approved.
Clinical Info
Provides genetic confirmation of APL. Predict relapse risk and monitor for minimal residual disease post-consolidation therapy.