Pneumococcal IgG Antibodies, 23 Serotypes




Test Mnemonic

PNE23

CPT Codes

  • 86317 - QTY (23)

Aliases

  • Streptococcus pneumoniae Antibodies

Includes

  • Streptococcus pneumoniae Serotype 1 Abs
  • Streptococcus pneumoniae Serotype 2 Abs
  • Streptococcus pneumoniae Serotype 3 Abs
  • Streptococcus pneumoniae Serotype 4 Abs
  • Streptococcus pneumoniae Serotype 5 Abs
  • Streptococcus pneumoniae Serotype 6B Abs
  • Streptococcus pneumoniae Serotype 7F Abs
  • Streptococcus pneumoniae Serotype 8 Abs
  • Streptococcus pneumoniae Serotype 9N Abs
  • Streptococcus pneumoniae Serotype 9V Abs
  • Streptococcus pneumoniae Serotype 10A Abs
  • Streptococcus pneumoniae Serotype 11A Abs
  • Streptococcus pneumoniae Serotype 12F Abs
  • Streptococcus pneumoniae Serotype 14 Abs
  • Streptococcus pneumoniae Serotype 15B Abs
  • Streptococcus pneumoniae Serotype 17F Abs
  • Streptococcus pneumoniae Serotype 18C Abs
  • Streptococcus pneumoniae Serotype 19A Abs
  • Streptococcus pneumoniae Serotype 19F Abs
  • Streptococcus pneumoniae Serotype 20 Abs
  • Streptococcus pneumoniae Serotype 22F Abs
  • Streptococcus pneumoniae Serotype 23F Abs
  • Streptococcus pneumoniae Serotype 33F Abs

Performing Laboratory

ARUP


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1.5 mLSerumSST (Gold) RefrigeratedSeparate serum from cells ASAP or within 2 hours of collection.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
0.3 mL     

Stability

Environmental Condition Description
AmbientAfter separation from cells: 48 hours
FrozenAfter separation from cells: 1 year (Avoid repeated freeze/thaw cycles)
RefrigeratedAfter separation from cells: 2 weeks

Days Performed

Tue, Fri

Turnaround Time

2 - 6 days

Methodology

Name Description
Quantitative Multiplex Bead Assay 

Reference Range

Special Info

Post-immunization specimens should be drawn 30 days after immunization. If both a pre-immunization and post-immunization specimen is collected, they must be received within 60 days of each other. Mark specimens clearly "PRE" or "POST" so that specimens will be saved and tested simultaneously.

Clinical Info

A pre- and post-vaccination comparison is required to adequately assess the humoral immune response to Prevnar 7 (P7), Prevnar 13 (P13), and/or Pneumovax 23 (PNX) Streptococcus pneumoniae vaccines. Pre-vaccination samples should be collected prior to vaccine administration. Post-vaccination samples should be obtained at least 4 weeks after immunization. Testing of post-vaccination samples alone will provide only general immune status of the individual to various pneumococcal serotypes. In the case of pure polysaccharide vaccine, indication of immune system competence is further delineated as an adequate response to at least 50 percent of the serotypes in the vaccine challenge for those 2-5 years of age and to at least 70 percent of the serotypes in the vaccine challenge for those 6-65 years of age. Individual immune response may vary based on age, past exposure, immunocompetence, and pneumococcal serotype. Responder Status Antibody Ratio Non-Responder . . . . . . . . . . . . . . Less than 2-fold Weak Responder . . . . . . . . . . . . . 2-fold to 4-fold Good Responder . . . . . . . . . . . . . Greater than 4-fold A response to 50-70 percent or more of the serotypes in the vaccine challenge is considered a normal humoral response1. Antibody concentration greater than 1.0 – 1.3 µg/mL is generally considered long-term protection2. References: 1. Daly TM, Pickering JW, Zhang X, Prince HE, Hill HR. Multilaboratory assessment of threshold versus fold-change algorithms for minimizing analytical variability in multiplexed pneumococcal IgG measurements. Clin Vaccine Immunol. 2014;21(7):982-8. 2. Daly TM, Hill HR. Use and Clinical Interpretation of Pneumococcal Antibody Measurements in the Evaluation of Humoral Immune Function. Clin Vaccine Immunol. 2015;22(2):148-152.