PNH Panel by FCM
- 88184 - QTY (1)
- 88185 - QTY (2)
- 88187 - QTY (1)
- Flow Panel for Paroxysmal Nocturnal Hemoglobinuria
Cleveland Clinic Laboratories
|Volume||Type||Container||Collect Temperature||Transport Temperature||Special Instructions|
Mon - Fri
1 - 3 days
|Flow Cytometry (FC)|
|PNH Panel by FCM|
|Sex||Age From||Age To||Type||Range||Range Unit|
|Freetext||Negative. No PNH clone detected.|
Do not draw on Fridays, weekends or holidays. The clinical significance of results on specimens 24-48 hours old should be evaluated in the context of other clinical and laboratory findings”. Specimens greater than 48 hours old will be rejected.
The presence of paroxysmal nocturnal hemoglobinuria (PNH) clones in the erythrocyte and granulocyte populations is assessed in this procedure. For erythrocytes antibodies to Glycophorin A are used to specifically gate red cells and PNH clones are identified by lack of CD59 expression. For granulocytes, CD15 and CD 33 are used to specifically gate granulocytes. The PNH-type granulocytes are then identified by lack of expression of CD24 and lack of reactivity to Fluorescent Aerolysin (FLAER). The lower limit of detection for this assay is 0.01% PNH-type cells. The presence of a PNH clone occurs in classical hemolytic PNH, generally at levels above 1%. PNH clones may be seen in other disorders such as aplastic anemia and myelodysplastic syndrome. Thus, these results must be put in context of the clinical findings.