PNH Panel by FCM




Test Mnemonic

PNHPNL

CPT Codes

  • 88184 - QTY (1)
  • 88185 - QTY (2)
  • 88187 - QTY (1)

LOINC ®

55164-8

Aliases

  • Flow Panel for Paroxysmal Nocturnal Hemoglobinuria

Includes

  • PNH Granulocyte clone
  • PNH RBC Clone-Partial Ag Loss (Type II)
  • PNH RBC Clone-Complete Ad Loss (Type III)
  • Sum of PNH RBC Clones (Type II + Type III)
  • Interpretation
  • Reviewed by

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
4 mLWhole bloodEDTA (Lavender) AmbientPeripheral blood samples to be delivered to the flow cytometry lab within 24 hours of draw time. Samples greater than 48 hours old will be rejected. Do not draw on Fridays, weekends or holidays.
4 mLWhole bloodEDTA (Lavender)RefrigeratedRefrigeratedPeripheral blood samples to be delivered to the flow cytometry lab within 24 hours of draw time. Samples greater than 48 hours old will be rejected. Do not draw on Fridays, weekends or holidays.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 mL     

Stability

Environmental Condition Description
Ambient48 hours
Refrigerated48 hours
FrozenUnacceptable

Days Performed

Mon - Fri

Turnaround Time

1 - 3 days

Methodology

Name Description
Flow Cytometry (FC) 

Reference Range

PNH Panel by FCM
Sex Age From Age To Type Range Range Unit
       Negative. No PNH clone detected. 

Special Info

Do not draw on Fridays, weekends or holidays. Specimens greater than 48 hours old will be rejected.

Clinical Info

The presence of paroxysmal nocturnal hemoglobinuria (PNH) clones in the erythrocyte and granulocyte populations is assessed in this procedure. For erythrocytes antibodies to Glycophorin A are used to specifically gate red cells and PNH clones are identified by lack of CD59 expression. For granulocytes, CD15 and CD 33 are used to specifically gate granulocytes. The PNH-type granulocytes are then identified by lack of expression of CD24 and lack of reactivity to Fluorescent Aerolysin (FLAER). The lower limit of detection for this assay is 0.01% PNH-type cells. The presence of a PNH clone occurs in classical hemolytic PNH, generally at levels above 1%. PNH clones may be seen in other disorders such as aplastic anemia and myelodysplastic syndrome. Thus, these results must be put in context of the clinical findings.