Protein Electrophoresis, Urine, with IFE




Test Mnemonic

UEPGRX

CPT Codes

  • 84156 - QTY (1)
  • 84166 - QTY (1)

Includes

  • Urine Total protein
  • % Albumin
  • % Alpha 1 globulin
  • % Alpha 2 globulin
  • % Beta globulin
  • % Gamma globulin
  • Interpretation

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
15 mLUrine, randomClean container AmbientFirst morning void preferred.

Alternate Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
15 mLUrine, 24-hour (well-mixed)Clean containerRefrigerate during collection.Refrigerated 

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
10 mL     

Stability

Environmental Condition Description
Ambient4 days
Refrigerated14 days
Frozen30 days

Days Performed

Mon - Fri

Turnaround Time

1 - 7 days

Methodology

Name Description
Electrophoresis 

Reference Range

Protein Electrophoresis, Urine, with IFE
Sex Age From Age To Type Range Range Unit
       No evidence of M-spike 

Special Info

Urine collected in acid container not acceptable. If indicated Monoclonal Protein analysis by immunofixation electrophoresis will be performed and charged.

Clinical Info

This test is used to screen for free monoclonal immunoglobulin light chains (Bence Jones proteins) and to evaluate renal disorders. However, immunofixation electrophoresis (IFE) is a more sensitive test for the detection of small M-proteins. A trace of albumin is usually seen in normal patients. Monoclonal free immunoglobulin light chains (Bence Jones proteins) and/or intact immunoglobulins may be seen in multiple myeloma and, much less commonly, in other B-cell lymphoproliferative disorders.