Synthetic Glucocorticoid Screen, Urine




Test Mnemonic

UGLUCO

CPT Codes

  • 80299 - QTY (1)

Includes

  • Prednisolone
  • Prednisone
  • Triamcinolone acetonide
  • Betamethasone
  • Budesonide
  • Dexamethasone
  • Fludrocortisone
  • Megestrol acetate
  • Methylprednisolone

Performing Laboratory

Mayo Clinic Dpt of Lab Med & Pathology


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
5 mLUrine, randomClean container Frozen 

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
0.6 mL     

Stability

Environmental Condition Description
AmbientUnacceptable
RefrigeratedUnacceptable
Frozen14 days

Days Performed

Wed

Turnaround Time

6 - 12 days

Methodology

Name Description
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) 
Stable Isotope Dilution 

Reference Range

Betamethasone
Sex Age From Age To Type Range Range Unit
       Negative 
Budesonide
Sex Age From Age To Type Range Range Unit
       Negative 
Dexmethasone
Sex Age From Age To Type Range Range Unit
       Negative 
Fludrocortisone
Sex Age From Age To Type Range Range Unit
       Negative 
Magestrol Acetate
Sex Age From Age To Type Range Range Unit
       Negative 
Methylprednisolone
Sex Age From Age To Type Range Range Unit
       Negative 
Prednisolone
Sex Age From Age To Type Range Range Unit
       Negative 
Prednisone
Sex Age From Age To Type Range Range Unit
       Negative 
Triamcinolone Acetonide
Sex Age From Age To Type Range Range Unit
       Negative 

Special Info

This test is New York State approved.

Clinical Info

This test is useful for confirming the presence of the listed synthetic glucocorticoids and the cause of secondary adrenal insufficiency. Cutoff Concentrations: Betamethasone: 0.10 mcg/dL; Budesonide: 0.10 mcg/dL; Dexamethasone: 0.10 mcg/dL; Fludrocortisone: 0.10 mcg/dL; Megestrol acetate: 0.10 mcg/dL; Methylprednisolone: 0.10 mcg/dL; Prednisolone: 0.10 mcg/dL; Prednisone: 0.10 mcg/dL; Triamcinolone acetonide: 0.10 mcg/dL. Lack of detection does not preclude use of synthetic glucocorticoid because adrenal suppression may persist for some time after the exogenous steroid is discontinued. This assay cannot detect all of the available synthetic steroids either available as pharmaceutical compounds or chemicals present in food. This assay confirms only the listed synthetic glucocorticoids.