Testosterone, Total and Free, Serum

Test Mnemonic


CPT Codes

  • 84402 - QTY (1)
  • 84403 - QTY (1)


  • Testosterone, Free, S
  • Testosterone, Total, S

Performing Laboratory

Mayo Clinic Dpt of Lab Med & Pathology

Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2.5 mLSerumNo additive (Red) RefrigeratedDo NOT draw serum gel tubes. Separate serum from cells and transfer to standard aliquot tube.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mL     


Environmental Condition Description
Refrigerated14 days
Frozen60 days

Days Performed

Mon - Sun

Turnaround Time

4 - 8 days


Name Description
Equilibrium Dialysis 
High Performance Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) 

Reference Range

Testosterone, Free
Sex Age From Age To Type Range Range Unit
Male1 Days15 DaysNormal0.20 - 3.10ng/dL
Male16 Days364 DaysNormalValues decrease gradually from newborn (0.20 - 3.10 ng/dL) to prepubertal levels. *Citation: J Clin Endocrinol Metab 1973;36(6):1132-1142.ng/dL
Male1 Years8 YearsNormal<0.13ng/dL
Male9 Years9 YearsNormal<0.13 - 0.45ng/dL
Male10 Years10 YearsNormal<0.13 - 1.26ng/dL
Male11 Years11 YearsNormal<0.13 - 5.52ng/dL
Male12 Years12 YearsNormal<0.13 - 9.28ng/dL
Male13 Years13 YearsNormal<0.13 - 12.6ng/dL
Male14 Years14 YearsNormal0.48 - 15.3ng/dL
Male15 Years15 YearsNormal1.62 - 17.7ng/dL
Male16 Years16 YearsNormal2.93 - 19.5ng/dL
Male17 Years17 YearsNormal4.28 - 20.9ng/dL
Male18 Years18 YearsNormal5.40 - 21.8ng/dL
Male19 Years19 YearsNormal5.36 - 21.2ng/dL
Male20 Years24 YearsNormal5.25 - 20.7ng/dL
Male25 Years29 YearsNormal5.05 - 19.8ng/dL
Male30 Years34 YearsNormal4.85 - 19.0ng/dL
Male35 Years39 YearsNormal4.65 - 18.1ng/dL
Male40 Years44 YearsNormal4.46 - 17.1ng/dL
Male45 Years49 YearsNormal4.26 - 16.4ng/dL
Male50 Years54 YearsNormal4.06 - 15.6ng/dL
Male55 Years59 YearsNormal3.87 - 14.7ng/dL
Male60 Years64 YearsNormal3.67 - 13.9ng/dL
Male65 Years69 YearsNormal3.47 - 13.0ng/dL
Male70 Years74 YearsNormal3.28 - 12.2ng/dL
Male75 Years79 YearsNormal3.08 - 11.3ng/dL
Male80 Years84 YearsNormal2.88 - 10.5ng/dL
Male85 Years89 YearsNormal2.69 - 9.61ng/dL
Male90 Years94 YearsNormal2.49 - 8.76ng/dL
Male95 Years100 YearsNormal2.29 - 7.91ng/dL
Female1 Days15 DaysNormal<0.13 - 0.25ng/dL
Female16 Days364 DaysNormalValues decrease gradually from newborn (<0.13 - 0.25 ng/dL) to prepubertal levels. *Citation: J Clin Endocrinol Metab 1973;36(6):1132-1142. ng/dL
Female1 Years4 YearsNormal<0.13ng/dL
Female5 Years5 YearsNormal<0.13ng/dL
Female6 Years6 YearsNormal<0.14ng/dL
Female7 Years7 YearsNormal<0.13 - 0.23ng/dL
Female8 Years8 YearsNormal<0.13 - 0.34ng/dL
Female9 Years9 YearsNormal<0.13 - 0.46ng/dL
Female10 Years10 YearsNormal<0.13 - 0.59ng/dL
Female11 Years11 YearsNormal<0.13 - 0.72ng/dL
Female12 Years12 YearsNormal<0.13 - 0.84ng/dL
Female13 Years13 YearsNormal<0.13 - 0.96ng/dL
Female14 Years14 YearsNormal<0.13 - 1.06ng/dL
Female15 Years18 YearsNormal<0.13 - 1.09ng/dL
Female19 Years19 YearsNormal<0.13 - 1.08ng/dL
Female20 Years24 YearsNormal<0.13 - 1.08ng/dL
Female25 Years29 YearsNormal<0.13 - 1.06ng/dL
Female30 Years34 YearsNormal<0.13 - 1.03ng/dL
Female35 Years39 YearsNormal<0.13 - 1.00ng/dL
Female40 Years44 YearsNormal<0.13 - 0.98ng/dL
Female45 Years49 YearsNormal<0.13 - 0.95ng/dL
Female50 Years54 YearsNormal<0.13 - 0.92ng/dL
Female55 Years59 YearsNormal<0.13 - 0.90ng/dL
Female60 Years64 YearsNormal<0.13 - 0.87ng/dL
Female65 Years69 YearsNormal<0.13 - 0.84ng/dL
Female70 Years74 YearsNormal<0.13 - 0.82ng/dL
Female75 Years79 YearsNormal<0.13 - 0.79ng/dL
Female80 Years84 YearsNormal<0.13 - 0.76ng/dL
Female85 Years89 YearsNormal<0.13 - 0.73ng/dL
Female90 Years94 YearsNormal<0.13 - 0.71ng/dL
Female95 Years100 YearsNormal<0.13 - 0.68ng/dL
Testosterone, Total
Sex Age From Age To Type Range Range Unit
Male  Months5 MonthsNormal75 - 400ng/dL
Male10 Years11 YearsNormal<7 - 130ng/dL
Male12 Years13 YearsNormal<7 - 800ng/dL
Male14 Years14 YearsNormal<7 - 1200ng/dL
Male15 Years16 YearsNormal100 - 1200ng/dL
Male17 Years18 YearsNormal300 - 1200ng/dL
Male19 Years99 YearsNormal240 - 950ng/dL
Male      NormalTanner Stage I (prepubertal): <7 - 20ng/dL
Male      NormalTanner Stage II: 8 - 66 ng/dL
Male      NormalTanner Stage III: 26 - 800ng/dL
Male      NormalTanner Stage IV: 85 - 1200ng/dL
Male      NormalTanner Stage V (young adult): 300 - 950ng/dL
       Male: 6 Months - 9 Years <7 - 20 ng/dL 
       Female: 6 Months - 9 Years <7 - 20 ng/dL 
       Note: Puberty onset (transition from Tanner Stage I to Tanner Stage II) occurs for boys at a median age of 11.5 (+/-) 2 years and for girls at a median age of 10.5 (+/-) 2 years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. For boys, there is no definite proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner Stages is variable. Tanner Stage V (young adult) should be reached by age 18. 
Female  Months5 MonthsNormal20 - 80ng/dL
Female10 Years11 YearsNormal<7 - 44ng/dL
Female12 Years16 YearsNormal<7 - 75ng/dL
Female17 Years18 YearsNormal20 - 75ng/dL
Female19 Years99 YearsNormal8 - 60ng/dL
Female      NormalTanner Stage I (prepubertal): <7 - 20 ng/dL
Female      NormalTanner Stage II: <7 - 47ng/dL
Female      NormalTanner Stage III: 17 - 75ng/dL
Female      NormalTanner Stage IV: 20 - 75ng/dL
Female      NormalTanner Stage V (young adult): 12 - 60ng/dL

Special Info

NOTE: Patient's age and sex are required. Serum gel tubes are NOT acceptable. Grossly hemolyzed, icteric or lipemic specimens will be rejected. This test is New York DOH approved.

Clinical Info

Useful as a second-level test for suspected increases or decreases in physiologically active testosterone. Indications: Assessment of androgen status in cases with suspected or known sex hormone-binding globulin-binding abnormalities; assessment of functional circulating testosterone in early pubertal boys and older men; assessment of functional circulating testosterone in women with symptoms or signs of hyperandrogenism, but normal total testosterone levels; monitoring of testosterone therapy or antiandrogen therapy in older men and in females. Cautions: Early-morning testosterone levels in young male individuals are 50% higher than p.m. levels, on average. Reference ranges have been derived from a.m. specimens. Testosterone levels can fluctuate substantially between different days, and sometimes even more rapidly. Assessment of androgen status should be based on more than a single measurement. The low end of the normal reference range for total testosterone in prepubertal subjects is not yet established. While free testosterone can be used for the same indications as bioavailable testosterone, determination of bioavailable testosterone levels may be superior to free testosterone measurement in most situations.