Testosterone, Total and Free, Serum | Cleveland Clinic Laboratories

Testosterone, Total and Free, Serum Print

Test Mnemonic

TFTEST

CPT Codes

84402 - QTY (1)
84403 - QTY (1)

Includes

Testosterone, Free, S
Testosterone, Total, S

Performing Laboratory

Mayo Clinic Dpt of Lab Med & Pathology

Specimen Requirements

Volume	Type	Container	Collect Temperature	Transport Temperature	Special Instructions
2.5 mL	Serum	No additive (Red)		Refrigerated	Do NOT draw serum gel tubes. Separate serum from cells and transfer to standard aliquot tube.

Minimum Specimen Requirements

Volume	Type	Container	Collect Temperature	Transport Temperature	Special Instructions
1 mL

Stability

Environmental Condition	Description
Refrigerated	14 days
Frozen	60 days
Ambient	Unacceptable

Days Performed

Mon - Sun

Turnaround Time

4 - 8 days

Methodology

Name	Description
Equilibrium Dialysis
High Performance Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

Reference Range

Testosterone, Free
Sex	Age From	Age To	Type	Range	Range Unit
Male	1 Days	15 Days	Normal	0.20 - 3.10	ng/dL
Male	16 Days	364 Days	Normal	Values decrease gradually from newborn (0.20 - 3.10 ng/dL) to prepubertal levels. *Citation: J Clin Endocrinol Metab 1973;36(6):1132-1142.	ng/dL
Male	1 Years	8 Years	Normal	<0.13	ng/dL
Male	9 Years	9 Years	Normal	<0.13 - 0.45	ng/dL
Male	10 Years	10 Years	Normal	<0.13 - 1.26	ng/dL
Male	11 Years	11 Years	Normal	<0.13 - 5.52	ng/dL
Male	12 Years	12 Years	Normal	<0.13 - 9.28	ng/dL
Male	13 Years	13 Years	Normal	<0.13 - 12.6	ng/dL
Male	14 Years	14 Years	Normal	0.48 - 15.3	ng/dL
Male	15 Years	15 Years	Normal	1.62 - 17.7	ng/dL
Male	16 Years	16 Years	Normal	2.93 - 19.5	ng/dL
Male	17 Years	17 Years	Normal	4.28 - 20.9	ng/dL
Male	18 Years	18 Years	Normal	5.40 - 21.8	ng/dL
Male	19 Years	19 Years	Normal	5.36 - 21.2	ng/dL
Male	20 Years	24 Years	Normal	5.25 - 20.7	ng/dL
Male	25 Years	29 Years	Normal	5.05 - 19.8	ng/dL
Male	30 Years	34 Years	Normal	4.85 - 19.0	ng/dL
Male	35 Years	39 Years	Normal	4.65 - 18.1	ng/dL
Male	40 Years	44 Years	Normal	4.46 - 17.1	ng/dL
Male	45 Years	49 Years	Normal	4.26 - 16.4	ng/dL
Male	50 Years	54 Years	Normal	4.06 - 15.6	ng/dL
Male	55 Years	59 Years	Normal	3.87 - 14.7	ng/dL
Male	60 Years	64 Years	Normal	3.67 - 13.9	ng/dL
Male	65 Years	69 Years	Normal	3.47 - 13.0	ng/dL
Male	70 Years	74 Years	Normal	3.28 - 12.2	ng/dL
Male	75 Years	79 Years	Normal	3.08 - 11.3	ng/dL
Male	80 Years	84 Years	Normal	2.88 - 10.5	ng/dL
Male	85 Years	89 Years	Normal	2.69 - 9.61	ng/dL
Male	90 Years	94 Years	Normal	2.49 - 8.76	ng/dL
Male	95 Years	100 Years	Normal	2.29 - 7.91	ng/dL
Female	1 Days	15 Days	Normal	<0.13 - 0.25	ng/dL
Female	16 Days	364 Days	Normal	Values decrease gradually from newborn (<0.13 - 0.25 ng/dL) to prepubertal levels. *Citation: J Clin Endocrinol Metab 1973;36(6):1132-1142.	ng/dL
Female	1 Years	4 Years	Normal	<0.13	ng/dL
Female	5 Years	5 Years	Normal	<0.13	ng/dL
Female	6 Years	6 Years	Normal	<0.14	ng/dL
Female	7 Years	7 Years	Normal	<0.13 - 0.23	ng/dL
Female	8 Years	8 Years	Normal	<0.13 - 0.34	ng/dL
Female	9 Years	9 Years	Normal	<0.13 - 0.46	ng/dL
Female	10 Years	10 Years	Normal	<0.13 - 0.59	ng/dL
Female	11 Years	11 Years	Normal	<0.13 - 0.72	ng/dL
Female	12 Years	12 Years	Normal	<0.13 - 0.84	ng/dL
Female	13 Years	13 Years	Normal	<0.13 - 0.96	ng/dL
Female	14 Years	14 Years	Normal	<0.13 - 1.06	ng/dL
Female	15 Years	18 Years	Normal	<0.13 - 1.09	ng/dL
Female	19 Years	19 Years	Normal	<0.13 - 1.08	ng/dL
Female	20 Years	24 Years	Normal	<0.13 - 1.08	ng/dL
Female	25 Years	29 Years	Normal	<0.13 - 1.06	ng/dL
Female	30 Years	34 Years	Normal	<0.13 - 1.03	ng/dL
Female	35 Years	39 Years	Normal	<0.13 - 1.00	ng/dL
Female	40 Years	44 Years	Normal	<0.13 - 0.98	ng/dL
Female	45 Years	49 Years	Normal	<0.13 - 0.95	ng/dL
Female	50 Years	54 Years	Normal	<0.13 - 0.92	ng/dL
Female	55 Years	59 Years	Normal	<0.13 - 0.90	ng/dL
Female	60 Years	64 Years	Normal	<0.13 - 0.87	ng/dL
Female	65 Years	69 Years	Normal	<0.13 - 0.84	ng/dL
Female	70 Years	74 Years	Normal	<0.13 - 0.82	ng/dL
Female	75 Years	79 Years	Normal	<0.13 - 0.79	ng/dL
Female	80 Years	84 Years	Normal	<0.13 - 0.76	ng/dL
Female	85 Years	89 Years	Normal	<0.13 - 0.73	ng/dL
Female	90 Years	94 Years	Normal	<0.13 - 0.71	ng/dL
Female	95 Years	100 Years	Normal	<0.13 - 0.68	ng/dL
Testosterone, Total
Sex	Age From	Age To	Type	Range	Range Unit
Male	Months	5 Months	Normal	75 - 400	ng/dL
Male	10 Years	11 Years	Normal	<7 - 130	ng/dL
Male	12 Years	13 Years	Normal	<7 - 800	ng/dL
Male	14 Years	14 Years	Normal	<7 - 1200	ng/dL
Male	15 Years	16 Years	Normal	100 - 1200	ng/dL
Male	17 Years	18 Years	Normal	300 - 1200	ng/dL
Male	19 Years	99 Years	Normal	240 - 950	ng/dL
Male			Normal	Tanner Stage I (prepubertal): <7 - 20	ng/dL
Male			Normal	Tanner Stage II: 8 - 66	ng/dL
Male			Normal	Tanner Stage III: 26 - 800	ng/dL
Male			Normal	Tanner Stage IV: 85 - 1200	ng/dL
Male			Normal	Tanner Stage V (young adult): 300 - 950	ng/dL
				Male: 6 Months - 9 Years <7 - 20 ng/dL
				Female: 6 Months - 9 Years <7 - 20 ng/dL
				Note: Puberty onset (transition from Tanner Stage I to Tanner Stage II) occurs for boys at a median age of 11.5 (+/-) 2 years and for girls at a median age of 10.5 (+/-) 2 years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. For boys, there is no definite proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner Stages is variable. Tanner Stage V (young adult) should be reached by age 18.
Female	Months	5 Months	Normal	20 - 80	ng/dL
Female	10 Years	11 Years	Normal	<7 - 44	ng/dL
Female	12 Years	16 Years	Normal	<7 - 75	ng/dL
Female	17 Years	18 Years	Normal	20 - 75	ng/dL
Female	19 Years	99 Years	Normal	8 - 60	ng/dL
Female			Normal	Tanner Stage I (prepubertal): <7 - 20	ng/dL
Female			Normal	Tanner Stage II: <7 - 47	ng/dL
Female			Normal	Tanner Stage III: 17 - 75	ng/dL
Female			Normal	Tanner Stage IV: 20 - 75	ng/dL
Female			Normal	Tanner Stage V (young adult): 12 - 60	ng/dL

Special Info

NOTE: Patient's age and sex are required. Serum gel tubes are NOT acceptable. Grossly hemolyzed, icteric or lipemic specimens will be rejected. This test is New York DOH approved.

Clinical Info

Useful as a second-level test for suspected increases or decreases in physiologically active testosterone. Indications: Assessment of androgen status in cases with suspected or known sex hormone-binding globulin-binding abnormalities; assessment of functional circulating testosterone in early pubertal boys and older men; assessment of functional circulating testosterone in women with symptoms or signs of hyperandrogenism, but normal total testosterone levels; monitoring of testosterone therapy or antiandrogen therapy in older men and in females. Cautions: Early-morning testosterone levels in young male individuals are 50% higher than p.m. levels, on average. Reference ranges have been derived from a.m. specimens. Testosterone levels can fluctuate substantially between different days, and sometimes even more rapidly. Assessment of androgen status should be based on more than a single measurement. The low end of the normal reference range for total testosterone in prepubertal subjects is not yet established. While free testosterone can be used for the same indications as bioavailable testosterone, determination of bioavailable testosterone levels may be superior to free testosterone measurement in most situations.