Trofile Co-receptor Tropism Assay
Test Mnemonic
TROFLE
CPT Codes
- 87999 - QTY (1)
Includes
- Tropotype Result
- Interpretation Note
- Activity of CCR5 antag anticipated?
Performing Laboratory
Mayo Clinic Dpt of Lab Med & Pathology
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3 mL | Plasma | EDTA PPT (White) | Frozen | Patient must have HIV RNA viral load >1000/copies/mL within 2 weeks prior to draw. Collect 2 EDTA (White) tubes. Separate plasma from cells ASAP or within six hours of collection and transfer into a standard aliquot tube and freeze. Separate specimens must be submitted when multiple tests are ordered. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3 mL | Plasma | EDTA (Lavender) | Frozen | Patient must have HIV RNA viral load >1000/copies/mL within 2 weeks prior to draw. Collect 2 EDTA (White) tubes. Separate plasma from cells ASAP or within six hours of collection and transfer into a standard aliquot tube and freeze. Separate specimens must be submitted when multiple tests are ordered. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Frozen | 14 days |
| Refrigerated | Unacceptable |
| Ambient | Unacceptable |
Days Performed
Sun - Sat
Turnaround Time
4 - 5 weeks
Methodology
| Name | Description |
|---|---|
| Polymerase Chain Reaction (PCR)/Culture |
Reference Range
Special Info
Order only if HIV RNA viral load, collected within the last 2 weeks, is >1000/copies/mL. The viral load and viral load collection date are required before the test will be performed. Collect 2 tubes. Separate plasma from cells ASAP or within six hours of collection and transfer into a standard aliquot tube and freeze. Separate specimens must be submitted when multiple tests are ordered. This test is New York DOH approved.
Clinical Info
Trofile (TM) is a CLIA-validated cell-based approach to determine an individual's HIV co-receptor tropism. Co-receptor tropism is defined as an interaction of a virus with a specific co-receptor on the target cell. To gain entry to the CD4+ cell (host), HIV must bind to the cell surface CD4 receptor and to one of two chemokine co-receptors (CCR5 or CXCR4) also present on the cell surface. Trofile can help determine which co-receptor antagonist, CCR5 or CXCR4, may be an appropriate drug for an individual patient.