Vitamin B1, Plasma




Test Mnemonic

PVITB1

CPT Codes

  • 84425 - QTY (1)

LOINC ®

2999-1

Aliases

  • Thiamine

Performing Laboratory

ARUP


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mLPlasmaSodium or Lithium heparin (Green) FrozenSeparate plasma from cells within 1 hour of collection. Transfer 1 mL plasma to a standard false-bottom aliquot tube. Note: Orange-capped tubes are not acceptable. Separate specimens must be submitted when multiple tests are ordered.

Alternate Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mLPlasmaEDTA (Lavender) FrozenSeparate plasma from cells within 1 hour of collection. Transfer 1 mL plasma to a standard false-bottom aliquot tube. Note: Orange-capped tubes are not acceptable. Separate specimens must be submitted when multiple tests are ordered.
1 mLPlasmaLithium heparin PST (Lt. Green) FrozenSeparate plasma from cells within 1 hour of collection. Transfer 1 mL plasma to a standard false-bottom aliquot tube. Note: Orange-capped tubes are not acceptable. Separate specimens must be submitted when multiple tests are ordered.
1 mLPlasmaSodium heparin PST [Lt. Green] FrozenSeparate plasma from cells within 1 hour of collection. Transfer 1 mL plasma to a standard false-bottom aliquot tube. Note: Orange-capped tubes are not acceptable. Separate specimens must be submitted when multiple tests are ordered.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
0.2 mL     

Stability

Environmental Condition Description
AmbientAfter separation from cells: Unacceptable
RefrigeratedAfter separation from cells: 1 week
FrozenAfter separation from cells: 6 months

Days Performed

Sun - Sat

Turnaround Time

3 - 5 days

Methodology

Name Description
High Performance Liquid Chromatography (HPLC) 

Reference Range

Vitamin B1, Plasma
Sex Age From Age To Type Range Range Unit
       Normal4-15nmol/L

Special Info

Do not use for evaluation of thiamine deficiency. Separate specimens must be submitted when multiple tests are ordered. Unacceptable conditions: Hemolyzed specimens or specimens other than heparin or EDTA plasma. This test is New York DOH approved.

Clinical Info

Thiamine (vitamin B1) is reported. However, thiamine diphosphate (TDP), the biologically active form of thiamine, is not found in measurable concentration in plasma, and is best determined in whole blood specimens. Plasma thiamine concentration reflects recent intake rather than body stores. Whole blood is the preferred specimen for thiamine assessment. Approximately 80% of thiamine present in whole blood is found in red blood cells.