Vitamin B1, Plasma
Test Mnemonic
PVITB1
CPT Codes
- 84425 - QTY (1)
LOINC ®
2999-1
Aliases
- Thiamine
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Plasma | Sodium or Lithium heparin (Green) | Frozen | Separate plasma from cells within 1 hour of collection. Transfer 1 mL plasma to a standard false-bottom aliquot tube. Note: Orange-capped tubes are not acceptable. Separate specimens must be submitted when multiple tests are ordered. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Plasma | EDTA (Lavender) | Frozen | Separate plasma from cells within 1 hour of collection. Transfer 1 mL plasma to a standard false-bottom aliquot tube. Note: Orange-capped tubes are not acceptable. Separate specimens must be submitted when multiple tests are ordered. | |
| 1 mL | Plasma | Lithium heparin PST (Lt. Green) | Frozen | Separate plasma from cells within 1 hour of collection. Transfer 1 mL plasma to a standard false-bottom aliquot tube. Note: Orange-capped tubes are not acceptable. Separate specimens must be submitted when multiple tests are ordered. | |
| 1 mL | Plasma | Sodium heparin PST [Lt. Green] | Frozen | Separate plasma from cells within 1 hour of collection. Transfer 1 mL plasma to a standard false-bottom aliquot tube. Note: Orange-capped tubes are not acceptable. Separate specimens must be submitted when multiple tests are ordered. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.2 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | After separation from cells: Unacceptable |
| Refrigerated | After separation from cells: 1 week |
| Frozen | After separation from cells: 6 months |
Days Performed
Sun - Sat
Turnaround Time
3 - 5 days
Methodology
| Name | Description |
|---|---|
| High Performance Liquid Chromatography (HPLC) |
Reference Range
| Vitamin B1, Plasma | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 4-15 | nmol/L |
Special Info
Do not use for evaluation of thiamine deficiency. Separate specimens must be submitted when multiple tests are ordered. Unacceptable conditions: Hemolyzed specimens or specimens other than heparin or EDTA plasma. This test is New York DOH approved.
Clinical Info
Thiamine (vitamin B1) is reported. However, thiamine diphosphate (TDP), the biologically active form of thiamine, is not found in measurable concentration in plasma, and is best determined in whole blood specimens. Plasma thiamine concentration reflects recent intake rather than body stores. Whole blood is the preferred specimen for thiamine assessment. Approximately 80% of thiamine present in whole blood is found in red blood cells.