Beginning in April 2018, there will be several testing changes, discontinuations, and additions:
Folate, Red Blood Cell (RBCFLP)
Aids in detecting folate deficiency.
82747 x 1
85014 x 1
Automated Cell Counter and Quantitative Chemiluminescent Immunoassay
Testing will be performed Sunday through Saturday.
Reporting time will be 2–3 days.
This test requires multiple specimen tubes.
Separate specimens must be submitted when multiple tests are ordered.
- Collect 1 ml whole blood in a Lavender BD Hemogard™ K2EDTA Tube.
- Protect tubes from light during collection, storage, and shipment.
- Mix specimen well, transfer into an amber transport tube, freeze, and transport frozen (critical).
- Collect 2.5 mL whole blood in a Lavender BD Hemogard™ K2EDTA Tube.
- Fill tube to at least half of fill volume, and send refrigerated.
- Note: Minimum volume is 1 mL split between 2 aliquots of 0.5 mL each.
Ambient: 2 hours
Refrigerated: 4 hours
Frozen: 2 months
Ambient: 24 hours
Refrigerated: 48 hours
≥ 366 ng/mL
0 – 14 Days: 39.6 – 57.2%
15 – 30 Days: 30.5 – 45.0%
31 – 60 Days: 26.8 – 37.5%
61 – 179 Days: 28.6 – 37.2%
6 – 23 Months: 30.8 – 37.9%
2 – 5 Years: 31.0 – 37.8%
6 – 11 Years: 32.2 – 39.8%
12 – 14 Years: 33.4 – 46.0%
15 – 99 Years (Male): 39.0 – 51.0%
15 – 99 Years (Female): 36.0 – 46.0%
- Hematocrit must be performed and submitted with the order.
- If the patient has not received a transfusion or experienced excessive bleeding between the RBC folate draw and the hematocrit draw, any hematocrit drawn within 24 hours of the RBC folate draw is acceptable.
- Folate RBC tube only: it is critical to submit the specimen as frozen; the specimen must be protected from light during collection, storage, and shipment.
- Separate specimens must be submitted when multiple tests are ordered.
- Specimens that are clotted or non-frozen will be rejected.
- This test is New York DOH approved.
Toxoplasmosis IgM and IgG, Ab: Equivocal results should have a new sample collected and tested 3 weeks later.
Rubella IgG Ab: If exposure to Rubella is suspected and a negative result is obtained, a second specimen should be collected and tested 1-2 weeks later. Equivocal results should have a second sample collected and tested.
Rubella IgM Ab: Parallel testing is preferred and convalescent specimens MUST be received within 30 days from receipt of acute specimens.
Herpes Simplex Type 1 and 2 IgG Antibodies: Equivocal results should be followed up by obtaining a new sample for re-testing.
Herpes Simplex IgM, Abs: A negative result does not rule out a primary or reactivated infection with HSV1 or HSV2 because the sample may have been obtained too early in the course of infection. If a primary infection is suspected, obtain a new sample 1-2 weeks later. Patients with equivocal results should have a new sample collected and tested 1-3 weeks later.
86644 x 1
86645 x 1
86694 x 1
86695 x 1
86696 x 1
86762 x 2
86777 x 1
86778 x 1
Chemiluminescence Immunoassay (CLIA)
Chemiluminescent Microparticle Immunoassay (CMIA)
Enzyme Immunoassay (EIA)
Semi-Quantitative Chemiluminescent Immunoassay
Refer to individual components.
After separation from cells:
Ambient: 8 hours
Refrigerated: 48 hours (note: For Rubella IgM: 2 weeks)
Frozen: 14 days
Refer to report.
Grossly hemolyzed, icteric, lipemic, and heat-inactivated samples are unacceptable.
Samples containing particulate matter or exhibiting obvious microbial contamination will be rejected.
EBV by PCR Qualitative (EBPCR)
EBPCR will be discontinued on April 12, 2018.
Discontinuation was originally scheduled for May 8 in the March 2018 Technical Update.
We suggest ordering EBV by PCR Quantitative (EBVQNT).