17-Hydroxyprogesterone Quantitative by HPLC-MS/MS, Timed Study

17-Hydroxyprogesterone Quantitative by HPLC-MS/MS, Timed Study Print

Test Mnemonic

HPROGT

CPT Codes

83498 - QTY (2)

Aliases

17-Hydroxyprogesterone, 60-Min
17-Hydroxyprogesterone, Baseline

Includes

17-Hydroxyprogesterone
17-Hydroxyprogesterone 60min

Performing Laboratory

ARUP

Specimen Requirements

Volume	Type	Container	Collect Temperature	Transport Temperature	Special Instructions
2 mL	Serum	SST (Gold)		Refrigerated	THIS TEST REQUIRES TWO SPECIMEN TUBES. Split sample into two 1 mL serum aliquots and refrigerate.

Alternate Specimen Requirements

Volume	Type	Container	Transport Temperature	Special Instructions
2 mL	Serum	No additive (Red)	Refrigerated	THIS TEST REQUIRES TWO SPECIMEN TUBES. Split sample into two 1 mL serum aliquots and refrigerate.
2 mL	Plasma	Lithium heparin PST (Lt. Green)	Refrigerated	THIS TEST REQUIRES TWO SPECIMEN TUBES. Split sample into two 1 mL plasma aliquots and refrigerate.
2 mL	Plasma	Sodium heparin PST [Lt. Green]	Refrigerated	THIS TEST REQUIRES TWO SPECIMEN TUBES. Split sample into two 1 mL plasma aliquots and refrigerate.
2 mL	Plasma	Sodium or Lithium heparin (Green)	Refrigerated	THIS TEST REQUIRES TWO SPECIMEN TUBES. Split sample into two 1 mL plasma aliquots and refrigerate.

Minimum Specimen Requirements

Volume	Type	Container	Collect Temperature	Transport Temperature	Special Instructions
0.6 mL					THIS TEST REQUIRES TWO SPECIMEN TUBES. Total minimum volume is 0.6 mL sample (split into two 0.3 mL aliquots).

Stability

Environmental Condition	Description
Refrigerated	After separation from cells: 1 week
Frozen	After separation from cells: 6 months
Ambient	After separation from cells: Unacceptable

Days Performed

Sun - Sat

Turnaround Time

2 - 6 days

Methodology

Name	Description
High Performance Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

Reference Range

17-Hydroxyprogesterone
Sex	Age From	Age To	Type	Range	Range Unit
				Female: Premature (26 - 28 weeks): 124-841 ng/dL
				Female: Premature (29 - 35 weeks): 26-568 ng/dL
				Female: Full term Day 3: 7 - 77 ng/dL
				Female: 6 Months to 1 Year: < or = 148 ng/dL
				Female: 18 Years and older: < 207 ng/dL
				Female (Follicular): 15-70 ng/dL
				Female (Luteal): 35-290 ng/dL
				Female: Tanner Stage I: < or = 74 ng/dL
				Female: Tanner Stage II: < or = 164 ng/dL
				Female: Tanner Stage III: 13-209 ng/dL
				Female: Tanner Stage IV-V: 7-170 ng/dL
				Male: Premature (26 - 28 weeks): 124-841 ng/dL
				Male: Premature (29 - 35 weeks): 26-568 ng/dL
				Male: Full term Day 3: 7-77 ng/dL
				Male: 18 Years and older: < 139 ng/dL
				Male: Tanner Stage I: < or = 62 ng/dL
				Male: Tanner Stage II: < or = 104 ng/dL
				Male: Tanner Stage III: < or = 151 ng/dL
				Male: 6 Months to 1 Year: < or = 148 ng/dL
				Male: Tanner Stage IV - V: 20-173 ng/dL
Female	16 Years	17 Years	Normal	< or = 178	ng/dL
Female	4 Days	30 Days	Normal	7-106	ng/dL
Female	1 Months	2 Months	Normal	13-106	ng/dL
Female	10 Years	12 Years	Normal	< or = 129	ng/dL
Female	13 Years	15 Years	Normal	9 - 208	ng/dL
Female	3 Months	5 Months	Normal	13-106	ng/dL
Female	2 Years	3 Years	Normal	< or = 256	ng/dL
Female	4 Years	6 Years	Normal	< or = 299	ng/dL
Female	7 Years	9 Years	Normal	< or = 71	ng/dL
Male	4 Days	30 Days	Normal	< 200	ng/dL
Male	1 Months	2 Months	Normal	< 200	ng/dL
Male	3 Months	5 Months	Normal	3 - 90	ng/dL
Male	2 Years	3 Years	Normal	< or = 228	ng/dL
Male	4 Years	6 Years	Normal	< or = 208	ng/dL
Male	7 Years	9 Years	Normal	< or = 63	ng/dL
Male	10 Years	12 Years	Normal	< or = 79	ng/dL
Male	13 Years	15 Years	Normal	9-140	ng/dL
Male	16 Years	17 Years	Normal	24-192	ng/dL

Special Info

Grossly hemolyzed specimens are unacceptable. This test is New York DOH approved.

Clinical Info

Note: Reference ranges for 17-Hydroxyprogesterone following stimulation are not well defined and are dependent on the stimulation method utilized.