17-Hydroxyprogesterone Quantitative by HPLC-MS/MS, Timed Study




Test Mnemonic

HPROGT

CPT Codes

  • 83498 - QTY (2)

Aliases

  • 17-Hydroxyprogesterone, 60-Min
  • 17-Hydroxyprogesterone, Baseline

Includes

  • 17-Hydroxyprogesterone
  • 17-Hydroxyprogesterone 60min

Performing Laboratory

ARUP


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 mLSerumSST (Gold) RefrigeratedTHIS TEST REQUIRES TWO SPECIMEN TUBES. Split sample into two 1 mL serum aliquots and refrigerate.

Alternate Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 mLSerumNo additive (Red) RefrigeratedTHIS TEST REQUIRES TWO SPECIMEN TUBES. Split sample into two 1 mL serum aliquots and refrigerate.
2 mLPlasmaLithium heparin PST (Lt. Green) RefrigeratedTHIS TEST REQUIRES TWO SPECIMEN TUBES. Split sample into two 1 mL plasma aliquots and refrigerate.
2 mLPlasmaSodium heparin PST [Lt. Green] RefrigeratedTHIS TEST REQUIRES TWO SPECIMEN TUBES. Split sample into two 1 mL plasma aliquots and refrigerate.
2 mLPlasmaSodium or Lithium heparin (Green) RefrigeratedTHIS TEST REQUIRES TWO SPECIMEN TUBES. Split sample into two 1 mL plasma aliquots and refrigerate.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
0.6 mL    THIS TEST REQUIRES TWO SPECIMEN TUBES. Total minimum volume is 0.6 mL sample (split into two 0.3 mL aliquots).

Stability

Environmental Condition Description
RefrigeratedAfter separation from cells: 1 week
FrozenAfter separation from cells: 6 months
AmbientAfter separation from cells: Unacceptable

Days Performed

Sun - Sat

Turnaround Time

2 - 6 days

Methodology

Name Description
High Performance Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) 

Reference Range

17-Hydroxyprogesterone
Sex Age From Age To Type Range Range Unit
       Female: Premature (26 - 28 weeks): 124-841 ng/dL 
       Female: Premature (29 - 35 weeks): 26-568 ng/dL 
       Female: Full term Day 3: 7 - 77 ng/dL 
       Female: 6 Months to 1 Year: < or = 148 ng/dL 
       Female: 18 Years and older: < 207 ng/dL 
       Female (Follicular): 15-70 ng/dL 
       Female (Luteal): 35-290 ng/dL 
       Female: Tanner Stage I: < or = 74 ng/dL 
       Female: Tanner Stage II: < or = 164 ng/dL 
       Female: Tanner Stage III: 13-209 ng/dL 
       Female: Tanner Stage IV-V: 7-170 ng/dL 
       Male: Premature (26 - 28 weeks): 124-841 ng/dL 
       Male: Premature (29 - 35 weeks): 26-568 ng/dL 
       Male: Full term Day 3: 7-77 ng/dL 
       Male: 18 Years and older: < 139 ng/dL 
       Male: Tanner Stage I: < or = 62 ng/dL 
       Male: Tanner Stage II: < or = 104 ng/dL 
       Male: Tanner Stage III: < or = 151 ng/dL 
       Male: 6 Months to 1 Year: < or = 148 ng/dL 
       Male: Tanner Stage IV - V: 20-173 ng/dL 
Female16 Years17 YearsNormal< or = 178ng/dL
Female4 Days30 DaysNormal7-106 ng/dL
Female1 Months2 MonthsNormal13-106 ng/dL
Female10 Years12 YearsNormal< or = 129ng/dL
Female13 Years15 YearsNormal9 - 208ng/dL
Female3 Months5 MonthsNormal13-106 ng/dL
Female2 Years3 YearsNormal< or = 256ng/dL
Female4 Years6 YearsNormal< or = 299ng/dL
Female7 Years9 YearsNormal< or = 71ng/dL
Male4 Days30 DaysNormal< 200 ng/dL
Male1 Months2 MonthsNormal< 200ng/dL
Male3 Months5 MonthsNormal3 - 90ng/dL
Male2 Years3 YearsNormal< or = 228ng/dL
Male4 Years6 YearsNormal< or = 208ng/dL
Male7 Years9 YearsNormal< or = 63ng/dL
Male10 Years12 YearsNormal< or = 79ng/dL
Male13 Years15 YearsNormal9-140ng/dL
Male16 Years17 YearsNormal24-192ng/dL

Special Info

Grossly hemolyzed specimens are unacceptable. This test is New York DOH approved.

Clinical Info

Note: Reference ranges for 17-Hydroxyprogesterone following stimulation are not well defined and are dependent on the stimulation method utilized.