Acute Leukemia NGS Panel, Bone Marrow




Test Mnemonic

HDMNGS

CPT Codes

  • 81455 - QTY (1)

Aliases

  • CEBPA
  • HDMNGS
  • NPM1
  • Hematologic Neoplasm Dual NGS, Bone Marrow

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
4 mLAspirate, bone marrowEDTA (Lavender)AmbientRefrigeratedRefrigerate ASAP

Stability

Environmental Condition Description
RefrigeratedStored up to 3 days. If testing is delayed, awaiting specimen triaging, store as NUCBUF
AmbientIf specimen is to be stored longer than 24 hours, it should be placed at 2-8 C for up to 3 days. If testing is delayed, awaiting specimen triaging, store as NUCBUF
FrozenUNACCEPTABLE

Days Performed

Mon - Fri

Turnaround Time

10 days

Methodology

Name Description
Next Gen Sequencing 

Special Info

RNA_genes: ABL1, ABL2, AFDN (MLLT4), ALK, BCL11B, BCL2, BCL3, BCL6, BCR, BIRC3, BLNK, CBFB, CBL, CCND1, CCND2, CCND3, CD274, CD28, CDK6, CDKN2A, CEBPA, CEBPD, CEBPE, CEBPG, CHD1, CHIC2, CIITA, CREBBP, CRLF2, CSF1R, CTLA4, DEK, DGKH, DUSP22, EBF1, EIF4A1, EPOR, ERG, ETV6, FGFR1, FLT3, FOXP1, GLIS2, HLF, ID4, IKZF1, IKZF2, IKZF3, IL2RB, IRF4, IRF8, ITK, JAK2, KAT6A, KLF2, KMT2A, LMO1, LMO2, LYN, MALT1, MECOM, MEF2D, MLF1, MLLT10, MRTFA (MKL1), MUC1, MYC, MYH11, NF1, NFKB2, NOTCH1, NTRK3, NUP214, NUP98, NUTM1, P2RY8, PAG1, PAX5, PBX1, PDCD1, PDCD1LG2, PDGFRA, PDGFRB, PICALM, PML, PRDM16, PTK2B, RARA, RBM15, ROS1, RUNX1, RUNX1T1, SEMA6A, SETD2, STIL, SYK, TAL1, TCF3, TFG, TLX1, TLX3, TP63, TSLP, TYK2, VAV1, ZCCHC7, ZNF384. DNA_genes: ABL1, ASXL1, BCOR, BCORL1, BRAF, CALR, CBL, CDKN2A, CEBPA, CSF3R, CUX1, DDX41, DNMT3A, EED, ETNK1, ETV6, EZH2, FBXW7, FLT3, GATA1, GATA2, GNAS, IDH1, IDH2, IKZF1, JAK2, JAK3, KDM6A, KIT, KMT2A, KRAS, LUC7L2, MPL, MYD88, NF1, NOTCH1, NPM1, NRAS, PAX5, PHF6, PIGA, PPM1D, PRPF8, PTEN, PTPN11, RAD21, RIT1, RUNX1, SETBP1, SF3B1, SH2B3, SMC1A, SMC3, SRSF2, STAG2, STAT3, STAT5B, SUZ12, TET2, TP53, U2AF1, WT1, ZRSR2

Clinical Info

DNA and RNA combination testing to evaluate for single nucleotide variants, small insertions and deletions, and common fusions in acute hematologic malignancies (acute myeloid and acute lymphoblastic leukemias); content includes all of the fusion targets of Hematologic Neoplasm Fusion NGS Panel as well as all of the DNA targets in the Chronic Myeloid NGS Panel.

Clinical Limitation

Not to be ordered concurrently with the Hematologic Neoplasm Fusion NGS Panel or the Chronic Myeloid NGS Panel, since this panel includes all targeted regions from those panels.