Benzodiazepines Confirmation, Urine




Test Mnemonic

UBENZC

CPT Codes

  • 80346 - QTY (1)
  • G0480 - QTY (1)

Includes

  • 7-aminoclonazepam
  • Alpha-hydroxyalprazolam
  • Alpha-hydroxytriazolam
  • Oxazepam
  • Lorazepam
  • Nordiazepam
  • Temazepam
  • Specimen Validity Creatinine
  • Specimen Validity pH
  • Specimen Validity Specific Gravity
  • Specimen Validity Oxidants

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 mLUrine, randomClean containerFrozen, ASAPFrozenSample must be frozen immediately after collection.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
0.5 mL    Sample must be frozen immediately after collection.

Stability

Environmental Condition Description
Ambient12 Hours at Room Temperature
Refrigerated12 Hours at 2 to 8 °C
Frozen7 Days at <-20 °C.

Days Performed

2 days per week

Turnaround Time

1 - 5 days

Methodology

Name Description
High Performance Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) 

Reference Range

7-aminoclonazepam, Urine
Sex Age From Age To Type Range Range Unit
   Years99 YearsNormal<40ng/mL
Alpha-hydroxyalprazolam, Urine
Sex Age From Age To Type Range Range Unit
   Years99 YearsNormal<60ng/mL
Alpha-hydroxytriazolam, Urine
Sex Age From Age To Type Range Range Unit
   Years99 YearsNormal<40ng/mL
Lorazepam, Urine
Sex Age From Age To Type Range Range Unit
   Years99 YearsNormal<40ng/mL
Nordiazepam, Urine
Sex Age From Age To Type Range Range Unit
   Years99 YearsNormal<40ng/mL
Oxazepam, Urine
Sex Age From Age To Type Range Range Unit
   Years99 YearsNormal<40ng/mL
Specimen Validity Chromate, Urine
Sex Age From Age To Type Range Range Unit
       Normal<50mg/L
Specimen Validity Creatinine, Urine
Sex Age From Age To Type Range Range Unit
       Normal20.0-300.0mg/dL
Specimen Validity Nitrites, Urine
Sex Age From Age To Type Range Range Unit
       Normal<500mg/L
Specimen Validity Oxidants, Urine
Sex Age From Age To Type Range Range Unit
       Normal<200mg/L
Specimen Validity pH, Urine
Sex Age From Age To Type Range Range Unit
       Normal4.5 - 8.0 
Specimen Validity Specififc Gravity, Urine
Sex Age From Age To Type Range Range Unit
       Normal1.003 - 1.035 
Temazepam, Urine
Sex Age From Age To Type Range Range Unit
   Years99 YearsNormal<40ng/mL

Special Info

Specimen Validity Testing (SVT) analytes have a stability of up to 5 days at 2-8°C as stated in the IFU. Based on the laboratory’s studies, this stability is based on samples that have reached an equilibrium. Introduction of oxidants to a urine sample will not be stable until an equilibrium is achieved. Time to reach equilibrium will vary dependent on the type and amount of oxidant used as an adulterant.

Clinical Info

For Specimen Validity Interpretations, the following rules are applied: Diluted: Creatinine is greater than or equal to 2mg/dL but less than 20mg/dL and Specific Gravity is greater than 1.0010 but less than 1.0030. Substituted: Creatinine is less than 2mg/dL and the Specific gravity is less than or equal to 1.0010 or greater than or equal to 1.0200. Adulterated: pH is less than 4 or greater than or equal to 11; Nitrite is greater than or equal to 500mg/L; Chromate is greater than or equal to 50mg/L; Oxidant is greater than or equal to 200mg/L.