Direct Immunofluorescence of Biopsy

CPT Codes

  • 88346 - QTY (1)


  • Cutaneous DIF
  • Lupus Band Test
  • Skin DIF

Performing Laboratory

Cleveland Clinic Laboratories

Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 - 4 mmBiopsySaline-moistened gauze in clean container RefrigeratedDeliver specimen to laboratory within 24 hours of collection. Refrigerate fresh specimens until transport. Indicate 'DIF' in special request area. Indicate patients name and unique identifier (MRN) on specimen container.

Days Performed

Mon - Fri

Turnaround Time

1 - 3 days


Name Description
Direct Immunofluorescence 

Reference Range

Special Info

Submit Surgical Pathology Requisition or accompany with an electronically generated test request. Specimen will be stained for IgG, IgA, IgM, CD3 and Fibrinogen; or, for kidney, albumin. Requisition must be completely filled out with date of procedure, patient's name, clinic number, date of birth or age, clinical history, clinician, procedure location, initial of nurse who assisted procedure, specimen submitted and any requests for special procedures (i.e. D.I.F.)

Clinical Info

Used to demonstrate in vivo fixation of antibody in autoimmune diseases. Interpretation by Pathologist.