FISH for 8;21 Translocation for AML Bone Marrow
Test Mnemonic
AMLFBM
CPT Codes
- 88237 - QTY (1)
- 88271 - QTY (2)
- 88275 - QTY (1)
- 88291 - QTY (1)
Performing Laboratory
n/a
Specimen Requirements
Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
---|---|---|---|---|---|
2 - 3 mL | Bone marrow | EDTA (Lavender) | Ambient | Ambient |
Alternate Specimen Requirements
Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
---|---|---|---|---|---|
2 - 3 mL | Bone marrow | Sodium heparin (Green) | Ambient | Ambient |
Stability
Environmental Condition | Description |
---|---|
Ambient | 48 hours |
Refrigerated | Acceptable |
Frozen | Not acceptable |
Days Performed
4 days per week
Turnaround Time
5 - 7 days
Methodology
Name | Description |
---|---|
Fluorescent In-Situ Hybridization (FISH) |
Clinical Info
Used in the diagnosis of Acute Myelogenous Leukemia. FISH analysis with the AML1 and ETO probes is valuable in cases of AML M2 because of its ability to reveal masked or variant, t(8;21) (q22;q22) translocations.