FISH for 8;21 Translocation for AML Bone Marrow




Test Mnemonic

AMLFBM

CPT Codes

  • 88237 - QTY (1)
  • 88271 - QTY (2)
  • 88275 - QTY (1)
  • 88291 - QTY (1)

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 - 3 mLBone marrowEDTA (Lavender)AmbientAmbient 

Alternate Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 - 3 mLBone marrowSodium heparin (Green)AmbientAmbient 

Stability

Environmental Condition Description
Ambient48 hours
RefrigeratedAcceptable
FrozenNot acceptable

Days Performed

4 days per week

Turnaround Time

5 - 7 days

Methodology

Name Description
Fluorescent In-Situ Hybridization (FISH) 

Clinical Info

Used in the diagnosis of Acute Myelogenous Leukemia. FISH analysis with the AML1 and ETO probes is valuable in cases of AML M2 because of its ability to reveal masked or variant, t(8;21) (q22;q22) translocations.