FISH for EGFR




Test Mnemonic

EGFRF

CPT Codes

  • 88377 - QTY (1)

Aliases

  • EGFR Amplification
  • Epidermal Growth Factor Receptor by FISH.

Includes

  • FISH for 7p12

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 slidesSlide (s), unstainedClean container AmbientOne formalin-fixed, paraffin embedded block containing representative tissue OR unstained 4 μm tissue sections on electrostatically charged slides containing representative tumor. Tissue must be fixed in formalin NOT B5 or PREFER fixative

Stability

Environmental Condition Description
AmbientFFPE tissue blocks and slides from FFPE specimens are stable at ambient temperatures

Days Performed

5 days per week

Turnaround Time

3 - 5 days

Methodology

Name Description
Fluorescent In-Situ Hybridization (FISH) 

Clinical Info

Interphase FISH analysis utilizing an enumeration probe for the EGFR gene at 7p11.2, as well as a probe specific to the centromere of chromosome 7, useful in the diagnosis of astrocytomas, oligodendrogliomas, ependymomas, or mixed gliomas. If no cells are available for analysis, no analysis charges will be incurred.