FISH for IGH/BCL2 Bone Marrow




Test Mnemonic

FSFCLM

CPT Codes

  • 88271 - QTY (2)
  • 88275 - QTY (1)
  • 88291 - QTY (1)
  • 88237 - QTY (1)

Aliases

  • FISH for t(14;18) translocation

Includes

  • LSI IGH (14q32)
  • LSI BCL2 (18q21)

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 - 3 mLBone marrowEDTA (Lavender)AmbientRefrigerated 

Alternate Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 - 3 mLBone marrowSodium heparin (Green)AmbientRefrigerated 

Stability

Environmental Condition Description
Ambient48 hours
RefrigeratedAcceptable
FrozenNot acceptable

Days Performed

5 days per week

Turnaround Time

3 - 5 days

Methodology

Name Description
Fluorescent In-Situ Hybridization (FISH) 

Clinical Info

This assay uses a dual color, dual fusion probe to detect the IGH/BCL2 translocation that is associated with follicular lymphoma and diffuse large B-cell lymphoma. This test should not be used to monitor minimal residual disease. If no cells are available for analysis, no analysis charges will be incurred.