Test Mnemonic


CPT Codes

  • 88377 - QTY (1)


  • FISH for 12q13-15
  • FISH for MDM2 Amplification


  • LSI 12q15
  • CEP12

Performing Laboratory

Cleveland Clinic Laboratories

Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 slidesParaffin block, formalin fixedClean container AmbientOne formalin-fixed, paraffin embedded block containing representative tissue OR unstained 4 μm tissue sections on electrostatically charged slides containing representative tumor. Tissue must be fixed in formalin NOT B5 or PREFER fixative


Environmental Condition Description
AmbientSample is formalin fixed and must not be fixed in Prefer or B5.

Days Performed

5 days per week

Turnaround Time

5 - 7 days


Name Description
Fluorescent In-Situ Hybridization (FISH) 

Reference Range

Clinical Info

Interphase FISH analysis utilizing an enumeration probe for the MDM2 gene at 12q15, as well as a probe for the centromere of chromosome 12. Identifying MDM2 gene amplification is useful in supporting a diagnosis of well-differentiated liposarcoma/atyplical lipomatous tumors. If no cells are available for analysis, no analysis charges will be incurred.