FISH for PDGFRA Blood




Test Mnemonic

PDGFRA

CPT Codes

  • 88271 - QTY (2)
  • 88275 - QTY (1)
  • 88291 - QTY (1)
  • 88237 - QTY (1)

Aliases

  • FISH for 4q12

Performing Laboratory

Cleveland Clinic Laboratories


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
5 - 7 mLBlood, peripheralEDTA (Lavender)AmbientAmbient 

Alternate Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
5 - 7 mLBlood, peripheralSodium heparin (Green)AmbientAmbient 

Stability

Environmental Condition Description
Ambient48 hours
RefrigeratedAcceptable
FrozenNot acceptable

Days Performed

3 days per week

Turnaround Time

5 - 7 days

Methodology

Name Description
Fluorescent In-Situ Hybridization (FISH) 

Reference Range

FISH for PDGFRA
Sex Age From Age To Type Range Range Unit
       Negative for PDGFRA rearrangement 

Clinical Info

This assay employs a tri-color probe to detect rearrangements of the PDGFRA locus on chromosome 4q12. Myeloid neoplasms with PDGFRA rearrangements are often associated with eosinophilia and represent a distinct clinicopathologic entity in the WHO classification of hematolymphoid neoplasms. This assay is useful for the evaluation of unexplained eosinophilia and to assist in the diagnosis and classification of myeloproliferative neoplasms.