FISH for PDGFRA Blood
Test Mnemonic
PDGFRA
CPT Codes
- 88271 - QTY (2)
- 88275 - QTY (1)
- 88291 - QTY (1)
- 88237 - QTY (1)
Aliases
- FISH for 4q12
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 5 - 7 mL | Blood, peripheral | EDTA (Lavender) | Ambient | Ambient |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 5 - 7 mL | Blood, peripheral | Sodium heparin (Green) | Ambient | Ambient |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 48 hours |
| Refrigerated | Acceptable |
| Frozen | Not acceptable |
Days Performed
3 days per week
Turnaround Time
5 - 7 days
Methodology
| Name | Description |
|---|---|
| Fluorescent In-Situ Hybridization (FISH) |
Reference Range
| FISH for PDGFRA | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Negative for PDGFRA rearrangement |
Clinical Info
This assay employs a tri-color probe to detect rearrangements of the PDGFRA locus on chromosome 4q12. Myeloid neoplasms with PDGFRA rearrangements are often associated with eosinophilia and represent a distinct clinicopathologic entity in the WHO classification of hematolymphoid neoplasms. This assay is useful for the evaluation of unexplained eosinophilia and to assist in the diagnosis and classification of myeloproliferative neoplasms.