HDL Cholesterol Subclasses




Test Mnemonic

HDLSUB

CPT Codes

  • 83701 - QTY (1)

Aliases

  • High Density Lipoprotein Cholesterol Subfractions

Includes

  • HDL2
  • HDL3 Cholesterol
  • HDL2/HDL3 Ratio

Performing Laboratory

Quest SJC


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
3 mLSerumNo additive (Red) RefrigeratedIf a cholesterol measurement is to be performed along with other lipid tests, the patient should fast 9-12 hours prior to collection.

Alternate Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
3 mLPlasmaSodium heparin (Green) RefrigeratedIf a cholesterol measurement is to be performed along with other lipid tests, the patient should fast 9-12 hours prior to collection.
3 mLSerumSST (Gold) RefrigeratedIf a cholesterol measurement is to be performed along with other lipid tests, the patient should fast 9-12 hours prior to collection.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1.5 mL     

Stability

Environmental Condition Description
Ambient1 day
Refrigerated1 week
Frozen2 weeks at -20C; 1 year at -70C

Days Performed

5 days per week

Turnaround Time

2 - 5 days

Methodology

Name Description
Colorimetry 
Enzymatic 
Precipitation 

Reference Range

HDL2 Cholesterol
Sex Age From Age To Type Range Range Unit
       Normal9 - 38mg/dL
HDL3 Cholesterol
Sex Age From Age To Type Range Range Unit
       Normal22 - 35mg/dL

Special Info

Patient Prep: If a cholesterol measurement is to be performed along with other lipid tests, the patient should fast 9-12 hours prior to collection.

Clinical Info

Evaluation of risk for coronary artery disease Elevations in triglycerides may affect analysis. Estrogen therapy and clofibrate (Atromid-S) will increase levels.