HLA-B27 PCR
Test Mnemonic
B27PCR
CPT Codes
- 81374 - QTY (1)
Aliases
- Ankylosing Spondylitis
- HLA-B27 Genotype
Performing Laboratory
Allogen Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 5 mL | Whole blood | EDTA (Lavender) | Ambient |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 7 mL | Whole blood | ACD A or B (Yellow) | Ambient | Collect one ACD A or B yellow top tube |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 5 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 1 week |
| Refrigerated | 1 week |
| Frozen | Unacceptable |
Days Performed
Mon - Fri
Turnaround Time
2 - 4 days
Methodology
| Name | Description |
|---|---|
| Polymerase Chain Reaction (PCR) | |
| Sequence Specific Oligonucleotide Probe (SSOP) |
Reference Range
Special Info
Transport WHOLE BLOOD at AMBIENT temperature. DO NOT CENTRIFUGE.
Clinical Info
HLA-B27 is strongly associated with ankylosing spondylitis (AS). HLA-B27 is also associated with other seronegative arthropathies such Reiter syndrome and psoriatic arthritis as well as extra-articular diseases such as anterior uveitis and inflammatory bowel disease. Greater than 90% of patients with AS are HLA-B27 positive. The frequency of HLA-B27 varies by ethnic group but generally <10 % in most US populations. HLA-B27 associated susceptibility to AS varies by population and HLA-B27 alleles detected. Some alleles such as B*27:05 are associated with high AS susceptibility while others such B*27:06 and B*27:09 are associated with low susceptibility. HLA-B27 allele typing is recommended in HLA-B27 positive cases.