IDH1 and IDH2 Hotspot PCR Analysis Tissue
Test Mnemonic
IDHID
CPT Codes
- 81120 - QTY (1)
- 81121 - QTY (1)
Aliases
- Isocitrate dehydrogenase 1
- Isocitrate dehydrogenase 2
- IDH 1 & 2
- IDH1-2
- IDH12
- IDH12G
Performing Laboratory
n/a
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 10 mm square | Block, Formalin fixed paraffin | Formalin-fixed, paraffin-embedded block | Ambient | Ambient | Formalin-fixed paraffin-embedded tissue slides. Transport and store slides at ambient temperature. Three unstained sections FFPE tissue on charged, unbaked slides or scrolls, plus one H&E stained section with best tumor area circled by a pathologist. Provide the percentage of tumor cells present, minimum of 10%. |
Stability
| Environmental Condition | Description |
|---|---|
| Refrigerated | Collect at ambient and transport at ambient or refrigerated temperature |
| Ambient | Collect and transport at ambient temperature |
| Frozen | Unacceptable |
Days Performed
Mon - Fri
Turnaround Time
3 days
Methodology
| Name | Description |
|---|---|
| Real-Time Polymerase Chain Reaction (RT-PCR) |
Special Info
n/a
Clinical Info
This assay looks for select hotspot mutations in IDH1 codon 132 and IDH2 codons 140 and 172
Clinical Limitation
n/a
Clinical Reference
n/a