Immunodeficiency (CDC) Panel | Cleveland Clinic Laboratories

Immunodeficiency (CDC) Panel Print

Test Mnemonic

IMMDEF

CPT Codes

86357 - QTY (1)
86359 - QTY (1)
86360 - QTY (1)
86355 - QTY (1)

Aliases

Lymphocyte Subsets

Includes

CD3+ T Cells
CD3+CD4+ T Cells
CD3+CD8+ T Cells
CD19+ B Cells
CD3-CD16/56+ NK Cells
CD4/CD8 Ratio

Performing Laboratory

Cleveland Clinic Laboratories

Specimen Requirements

Volume	Type	Container	Collect Temperature	Transport Temperature	Special Instructions
4 mL	Whole blood	EDTA (Lavender)	Ambient	Ambient	Deliver specimens to testing laboratory within 24 hours of collection.

Minimum Specimen Requirements

Volume	Type	Container	Collect Temperature	Transport Temperature	Special Instructions
1 mL

Stability

Environmental Condition	Description
Ambient	Deliver specimens to testing laboratory within 24 hours of collection.
Refrigerated	Unacceptable
Frozen	Unacceptable

Days Performed

Mon - Sat

Turnaround Time

1 - 2 days

Reference Range

CD19+ B Cell %
Sex	Age From	Age To	Type	Range	Range Unit
	5 Years	99 Years	Normal	5 - 22	%
CD19+ B Cell No.
Sex	Age From	Age To	Type	Range	Range Unit
	5 Years	99 Years	Normal	75 - 660	cells/uL
CD3+ T Cell %
Sex	Age From	Age To	Type	Range	Range Unit
	Months	3 Months	Normal	60 - 87	%
	4 Months	11 Months	Normal	57 - 84	%
	12 Months	23 Months	Normal	53 - 81	%
	24 Months	59 Months	Normal	62 - 80	%
	5 Years	99 Years	Normal	60 - 89	%
CD3+ T Cell No.
Sex	Age From	Age To	Type	Range	Range Unit
	Months	3 Months	Normal	2070 - 6540	cells/uL
	4 Months	11 Months	Normal	2280 - 6450	cells/uL
	12 Months	23 Months	Normal	1460 - 5440	cells/uL
	24 Months	59 Months	Normal	1610 - 4230	cells/uL
	5 Years	99 Years	Normal	958 - 2388	cells/uL
CD3+CD8+ T Cell %
Sex	Age From	Age To	Type	Range	Range Unit
	Months	3 Months	Normal	16 - 35	%
	4 Months	11 Months	Normal	16 - 34	%
	12 Months	23 Months	Normal	16 - 38	%
	24 Months	59 Months	Normal	22 - 38	%
	5 Years	99 Years	Normal	10 - 41	%
CD3+CD8+ T Cell No.
Sex	Age From	Age To	Type	Range	Range Unit
	Months	3 Months	Normal	650 - 2450	cells/uL
	4 Months	11 Months	Normal	720 - 2490	cells/uL
	12 Months	23 Months	Normal	570 - 2230	cells/uL
	24 Months	59 Months	Normal	630 - 1910	cells/uL
	5 Years	99 Years	Normal	175 - 958	cells/uL
CD4/CD8 Ratio
Sex	Age From	Age To	Type	Range	Range Unit
	Months	3 Months	Normal	1.32 - 3.47	.
	4 Months	11 Months	Normal	1.20 - 3.48	.
	12 Months	23 Months	Normal	0.95 - 2.95	.
	24 Months	59 Months	Normal	1.05 - 2.07	.
	5 Years	99 Years	Normal	1.10 - 3.25	.
CD4+CD3+ T Cell %
Sex	Age From	Age To	Type	Range	Range Unit
	Months	3 Months	Normal	41 - 64	%
	4 Months	11 Months	Normal	36 - 61	%
	12 Months	23 Months	Normal	31 - 54	%
	24 Months	59 Months	Normal	25 - 51	%
	5 Years	99 Years	Normal	34 - 61	%
CD4+CD3+ T Cell No.
Sex	Age From	Age To	Type	Range	Range Unit
	Months	3 Months	Normal	1460 - 5110	cells/uL
	4 Months	11 Months	Normal	1690 - 4600	cells/uL
	12 Months	23 Months	Normal	1020 - 3600	cells/uL
	24 Months	59 Months	Normal	900 - 2860	cells/uL
	5 Years	99 Years	Normal	533 - 1674	cells/uL
NK Cell %
Sex	Age From	Age To	Type	Range	Range Unit
	5 Years	99 Years	Normal	102 - 565	cells/uL
	5 Years	99 Years	Normal	5 - 25	%

Special Info

Samples received between 24 and 48 hours of draw time will be performed and reported along with a lymphocyte viability. Samples received between 48 and 72 hours old will performed and reported along with a lymphocyte viability; however, NK results may not be accurate. Recommend obtaining a fresh sample if clinically indicated. Samples that are less than 75% viable will be rejected and a redraw will be requested. Samples received in testing lab beyond 72 hours cannot be performed. Refrigerated samples may give abberant results and will be rejected.

Clinical Info

This is a single platform, 6-color Flow Cytometry assay used to enumerate CD3+ T cells; CD3+CD4+ T cells; CD3+CD8+ T cells; CD19+ B cells; CD3-CD16/56+ NK cells; and CD4/CD8 Ratio in peripheral blood. These lymphocyte subset percentages and counts are used in the monitoring of Immunodeficiency, HIV infection, and other immunologic disorders.