KRAS Hotspot PCR Analysis
Test Mnemonic
KRASID
CPT Codes
- 81275 - QTY (1)
- 81276 - QTY (1)
- ALT Code 81479 - QTY (1)
Aliases
- KRAS
- KRASGN
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 10 mm square | Block, Formalin fixed paraffin | Formalin-fixed, paraffin-embedded block | Ambient | Ambient | Formalin-fixed paraffin-embedded tissue slides. Transport and store slides at ambient temperature. Three unstained sections FFPE tissue on charged, unbaked slides or scrolls, plus one H&E stained section with best tumor area circled by a pathologist. Provide the percentage of tumor cells present, minimum 10%. |
| 10 mm square | Cell block, formalin fixed paraffin | Formalin-fixed, paraffin-embedded block | Ambient | Ambient | Formalin-fixed paraffin-embedded cell block slides. Transport and store slides at ambient temperature. Three unstained sections FFPE cell block tissue on charged, unbaked slides. Provide the percentage of tumor cells present, minimum 10%. |
| 10 mm square | Aspirate, fine needle | Slide | Ambient | Ambient | One alcohol fixed cytology slide. Tissue adequacy must be assessed by cytopathologist. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | FFPET slides and scrolls, cell block slides and alcohol fixed slides are stable at ambient temperature indefinitely. |
| Refrigerated | FFPET slides and scrolls, cell block slides and alcohol fixed slides are stable at ambient temperature indefinitely. |
| Frozen | Unacceptable. |
Days Performed
Mon - Fri
Turnaround Time
3 days
Methodology
| Name | Description |
|---|---|
| Real-Time Polymerase Chain Reaction (RT-PCR) |
Special Info
Detects 21 known mutations of the KRAS gene.
Clinical Limitation
Detects 21 known mutations of the KRAS gene