Liver Fibrosis, FibroTest-ActiTest




Test Mnemonic

LIVFIB

CPT Codes

  • 81596 - QTY (1)

LOINC ®

Not available

Aliases

  • FibroTest
  • Liver Acti
  • Liver Fibrosis
  • Necroinflammatory Activity Score

Includes

  • Alanine aminotransferase (ALT)
  • Alpha 2-Macroglobulin
  • Apolipoprotein A1
  • Bilirubin, Total
  • Gamma-glutamyl Transferase (GGT)
  • Haptoglobin
  • Fibrosis Score
  • Necroinflammat Act Score

Performing Laboratory

n/a


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 mLSerumSST (Gold) RefrigeratedCentrifuge within 2 hours from collection. Protect serum from light after centrifugation. Overnight fasting is preferred.

Alternate Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
2 mLSerumNo additive (Red) RefrigeratedCentrifuge within 2 hours from collection. Transfer serum into a standard or amber aliquot tube. Protect serum from light after centrifugation. Overnight fasting is preferred.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mL     

Stability

Environmental Condition Description
Ambient24 hours
Refrigerated7 days
Frozen-20 C: Not acceptable; -70 C: 28 days

Days Performed

Mon - Sat

Turnaround Time

2 - 5 days

Methodology

Name Description
Spectrophotometry (S) 
Colorimetric Enzyme Assay 
Immunoturbidometric Assay 

Reference Range

Alpha 2 Macroglobulin
Sex Age From Age To Type Range Range Unit
Male      Normal80-290mg/dL
Female      Normal110-270mg/dL
ALT
Sex Age From Age To Type Range Range Unit
Male      Normal10-50U/L
Female      Normal10-35U/L
Apolipoprotein A1
Sex Age From Age To Type Range Range Unit
 2 Years17 YearsNormal>120mg/dL
Male18 Years99 YearsNormal>114mg/dL
Female18 Years99 YearsNormal>124mg/dL
Bilirubin, Total
Sex Age From Age To Type Range Range Unit
       Normal0.2-1.3mg/dL
Fibrosis Interpretation
Sex Age From Age To Type Range Range Unit
        FibroTest Score: >=0 and <=0.21 - Metavir Score: F0 no fibrosis 
       FibroTest Score: >0.21 and <=0.27 - Metavir Score: F0-F1 no fibrosis 
        FibroTest Score: >0.27 and <=0.31 - Metavir Score: F1 minimal fibrosis 
       FibroTest Score: >0.31 and <=0.48 - Metavir Score: F1-F2 minimal fibrosis 
       FibroTest Score: >0.48 and <=0.58- Metavir Score: F2 moderate fibrosis 
       FibroTest Score: >0.58 and <=0.72 - Metavir Score: F3 advanced fibrosis 
       FibroTest Score: >0.72 and <=0.74 - Metavir Score: F3-F4 advanced fibrosis 
       FibroTest Score: >0.74 and <=1.00- Metavir Score: F4 severe fibrosis 
Fibrosis Score
Sex Age From Age To Type Range Range Unit
       No reference range available 
Fibrosis Stage
Sex Age From Age To Type Range Range Unit
       No reference range available 
GGT
Sex Age From Age To Type Range Range Unit
Male      Normal10-71U/L
Female      Normal6-42U/L
Haptoglobin
Sex Age From Age To Type Range Range Unit
       Normal31-238mg/dL
Necroinflammatory Activity Grade
Sex Age From Age To Type Range Range Unit
       No reference range available 
Necroinflammatory Activity Interpretation
Sex Age From Age To Type Range Range Unit
       ActiTest Score: >=0 and <=0.17 - Metavir Score: A0 no activity 
       ActiTest Score: >0.17 and <=0.29 - Metavir Score: A0-A1 no activity 
       ActiTest Score: >0.29 and <=0.36 - Metavir Score: A1 minimal activity 
       ActiTest Score: >0.36 and <=0.52 - Metavir Score: A1-A2 minimal activity 
       ActiTest Score: >0.52 and <=0.60 - Metavir Score: A2 significant activity 
       ActiTest Score: >0.60 and <=0.62 - Metavir Score: A2-A3 significant activity 
       ActiTest Score: >0.62 and <=1.00 - Metavir Score: A3 severe activity 
Necroinflammatory Activity Score
Sex Age From Age To Type Range Range Unit
       No reference range available 

Special Info

Protect from light. Avoid freeze/thaw cycles of specimen. Overnight fasting is preferred. Grossly hemolyzed and grossly lipemic specimens will be rejected. Specimens from patients less than 2 years old are unacceptable. Additionally, specimens QNS for one or more analytes will not be accepted.

Clinical Info

The FibroTest-ActiTest interpretation is a proprietary algorithm owned by BioPredictive. FibroTest and ActiTest permit the non-invasive evaluation of Hepatitis B or C individuals for the presence of liver fibrosis and liver inflammation, respectively. The FibroTest and ActiTest scores are calculated based on patient age, gender and concentrations of serum of y-glutamyl transferase (GGT), total bilirubin (TB), a-2 macroglobulin, haptoglobin, apolipoprotein A1 and alanine aminotransferase (ActiTest). Fibrotest and ActiTest Scores, on a scale of 0.0 to 1.0, are assigned a Metavir scale indicating the level of fibrosis or inflammation present.

Clinical Limitation

Specimens from patients less than 2 years of age are unacceptable.