Protein Electrophoresis, Serum, with IFE
Test Mnemonic
SEPGRX
CPT Codes
- 84165 - QTY (1)
- 84155 - QTY (1)
LOINC ®
55295-0
Includes
- Total protein
- Albumin
- Alpha-1 globulin
- Alpha-2 globulin
- Beta globulin
- Gamma globulin
- M protein location
- M protein gm/dL
- Interpretation
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Serum | SST (Gold) | Refrigerated |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.5 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 4 days |
| Refrigerated | 14 days |
| Frozen | 30 days |
Days Performed
Mon - Fri
Turnaround Time
1 - 7 days
Methodology
| Name | Description |
|---|---|
| Capillary Electrophoresis (CE) |
Reference Range
| Albumin | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 3.43 – 5.41 | g/dL | |||
| Alpha-1 globulin | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 0.18 - 0.43 | g/dL | |||
| Alpha-2 globulin | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 0.42 – 0.98 | g/dL | |||
| Beta globulin | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 0.61 – 1.17 | g/dL | |||
| Gamma globulin | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 0.53 – 1.51 | g/dL | |||
| M Spike Concentration | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 0.00 | g/dL | |||
| Total protein | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| 18 Years | 99 Years | Normal | 6.3 - 8.0 | g/dL |
Special Info
Avoid hemolysis. If indicated, Monoclonal Protein analysis by immunofixation electrophoresis will be performed and charged.
Clinical Info
Serum protein electrophoresis is useful as a screening procedure in the detection of monoclonal gammopathies. If an M protein is identified, it will be reflexed for confirmation using immunofixation electrophoresis (IFE).