Targeted Oncology Panel Next Generation Sequencing Cytology
Test Mnemonic
TOPCY
CPT Codes
- 81455 - QTY (1)
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 10 | Cell block, alcohol fixed | Slide | Ambient | Ambient | 10 slides from a cell block and 1 H&E stained slide |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 10 | Cell block, formalin fixed paraffin | Slide | Ambient | Ambient | 10 slides from a cell block |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Stable at ambient temperature for 48 hours. |
| Refrigerated | Refrigerate at 2- 8C for up to 7 days. |
| Frozen | Unacceptable |
Days Performed
3 days per week
Turnaround Time
8 days
Special Info
The following genes are interrogated: ABL1, AKT, AKT1, AKT3, AR, AXL, BRAF, C11orf95, CCND1, CDK4, CDK6, CTNNB1, DDR2, EGFR, ERBB2, ERBB3, ERBB4, ERG, ESR1, ETV1, ETV4, ETV5, FGFR1, FGFR2, FGFR3, FGFR4, GNA11, GNAQ, GNAS, H3-3A, H3-3B, HRAS, IDH1, IDH2, JAK1, JAK2, JAK3, KIT, KRAS, MAP2K1, MAP2K2, MET, MTOR, MYC, MYCN, NRAS, NTRK1, NTRK2, NTRK3, PDGRFA, PIK3CA, PPARG, RAF1, RELA, RET, ROS1, SMO, TERT AND TP53.
Clinical Info
This pan-cancer assay evaluates tumor hotspots, SNVs, indels, CNVs, and gene fusions from DNA and RNA. This information can be used for diagnostic, prognostic and therapeutic purposes across a variety of solid and liquid tumors.