TMA Complement Panel
Test Mnemonic
TMAPAN
CPT Codes
- 86160 - QTY (1)
- 86160 - QTY (1)
- 86160 - QTY (1)
- 83516 - QTY (1)
- 86160 - QTY (1)
- 86160 - QTY (1)
Aliases
- aHUS
- Atypical Hemolytic Uremic Syndrome
- Thrombotic Microangiopathy
Performing Laboratory
Children's Hosp of Cincinnati
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Plasma | Lithium heparin PST (Lt. Green) | Centrifuge and refrigerate. | Multiple specimen tubes must be collected for this panel. This tube is for test: Complement C 3 (C3COMP) and Complement C4 (C4COMP). | |
| 4 mL | Serum | SST (Gold) | Frozen | Multiple specimen tubes must be collected for this panel. This tube is for test: Complement Factor B (COMPFB), Complement Factor H Autoantibody (CMPFHA), Complement Factor H (COMPFH), and Complement Factor I (COMPFI). Separate serum from cells ASAP or within 2 hours of collection. Transfer serum to standard aliquot tube and freeze. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Serum | SST (Gold) | Centrifuge and refrigerate. | Multiple specimen tubes must be collected for this panel. This tube is for test: Complement C 3 (C3COMP) and Complement C4 (C4COMP). |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1.8 mL | Submitting the minimum volume will not allow for repeat testing or add-ons. The aliquot for Complement C 3 (C3COMP) and Complement C4 (C4COMP) must have a minimum volume of 0.8 mL. The aliquot for Complement Factor B (COMPFB), Complement Factor H Autoantibody (CMPFHA), Complement Factor H (COMPFH), and Complement Factor I (COMPFI) must have a minimum volume of 1.0 mL. |
Stability
| Environmental Condition | Description |
|---|---|
| Frozen | After separation from cells: 2 mL aliquot for C3COMP/C4COMP: Unacceptable; 4 mL aliquot for COMPFB/CMPFHA/COMPFH/COMPFI: Acceptable |
| Refrigerated | After separation from cells: 2 mL aliquot for C3COMP/C4COMP: 48 hours; 4 mL aliquot for COMPFB/CMPFHA/COMPFH/COMPFI: Unacceptable |
| Ambient | After separation from cells: 2 mL aliquot for C3COMP/C4COMP: 48 hours; 4 mL aliquot for COMPFB/CMPFHA/COMPFH/COMPFI: Unacceptable |
Days Performed
Varies
Turnaround Time
1 - 16 days
Methodology
| Name | Description |
|---|---|
| Enzyme-Linked Immunosorbent Assay (ELISA) | |
| Radial Immunodiffusion (RID) | |
| Turbidimetric Immunoassay (TUI) |
Special Info
Performing Labs: Automated Chemistry (x45476), Client Services (x45755) - Vendor Children's Hosp of Cincinnati. Refer to individual tests: Complement C 3 (C3COMP), Complement C4 (C4COMP), Complement Factor B (COMPFB), Complement Factor H Autoantibody (CMPFHA), Complement Factor H (COMPFH) and Complement Factor I (COMPFI).